To evaluate the effect of orthodontic appliances on physicochemical, biochemical, and oxidative stress changes in salivary parameters during treatment.

Materials and Methods

A cohort study was conducted with 112 healthy patients. Salivary samples were taken at baseline, 1 month, and 9 months after placement of the orthodontic appliances used in treatment.


A statistically significant difference was observed in certain examined salivary parameters, including enzymes, electrolytes, and oxidative stress markers.


The use of aligners had a lower prevalence of disturbing salivary parameters. Orthodontist must consider these changes to prevent the occurrence of white spot lesions.

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Author notes


Professor, Laboratory of Oral Health and Orofacial Rehabilitation, Orthodontic Department, University of Monastir, Tunisia.


Assistant Professor, Laboratory for Research on Biologically Compatible Compounds, Faculty of Dental Medicine, University of Monastir; Medicine Faculty of Sousse, University of Sousse, Tunisia.


Laboratory of Transmissible Diseases and Biologically Active Substances, University of Monastir, Tunisia; University of Tunis El Manar, Faculty of Medicine of Tunis, Department of Basic Sciences, Tunisia.


Private practice, Monastir, Tunisia.


Professor, Laboratory of Biochemistry-Toxicology, University of Monastir, Tunisia.


Professor, Laboratory of Infectious Diseases and Biological Agents, Monastir, Tunisia.