Identifying threats and their regional occurrence across a species’ range is increasingly valuable for prioritising threat-specific interventions and achieving effective conservation outcomes. We surveyed registered wildlife rehabilitators to identify (i) threats faced by the koala across Victoria and (ii) their perceptions on koala population trends and potential threat mitigation actions. Wildlife rehabilitators identified habitat loss, fragmentation, and degradation as the biggest threat to koalas, while vehicle collisions, heatwaves and wildfire were also identified as key threats. Accordingly, reducing the clearing of native vegetation was considered the most effective threat mitigation action, while creating of wildlife corridors, planting of more food trees, and educating communities living in koala occupied areas were also considered appropriate mitigation strategies. Finally, 89% of wildlife rehabilitators believed that koala numbers are declining in their region.