Of the 13 films listed in the catalogue of motion picture films of the Thylacine (Sleightholme & Campbell, 2021a), two taken at the Beaumaris Zoo on the Queen’s Domain (Hobart) were noted as being lost; the 1924 Cook film of the Mullins family group and the 1931 Stewart film of the last evidentially proven captive Thylacine taken soon after its arrival at the zoo (Linnard & Sleightholme, 2023). Inspired by mention of the existence of the Stewart film in Sleightholme et al., (2020) and a direct appeal to find the missing films in Sleightholme & Campbell (2021a), a Greek Thylacine enthusiast successfully located the Thylacine content of the missing Stewart film in January 2022. This was comprised of two parts – one of the Thylacine in its enclosure and the other of it pacing in the background behind a Tasmanian devil (Sarcophilus harrisii) in the adjacent enclosure, the only occasion where both species were captured together on film. The provenance of the Stewart film is discussed, its subject identified, and key stills from the two recovered sequences are reproduced here for the first time.

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