Tree-kangaroos Dendrolagus sp. are a poorly known group of folivorous arboreal macropodid marsupials endemic to the rainforests of north-eastern Australia and New Guinea. Over the last century there has been little agreement on the relationship between the two Australian species, D. lumholtzi and D. bennettianus. In light of this uncertainty, we undertook a phylogenetics study based on 430 bp of sequence from the mitochondrial DNA gene cytochrome b (cytb). Samples were collected from D. lumholtzi (n = 45) and D. bennettianus (n = 3), as well as representative of six New Guinean Dendrolagus species. Results from distance and parsimony analyses strongly support a sister relationship between the two Australian species. However, D. lumholtzi and D. bennettanius show considerable sequence divergence (5.3%), suggesting that their speciation predates the mid-late Pleistocene. Although inter-relationships amongst New Guinean Dendrolagus were not fully resolved by our preliminary analysis, a number of distinct lineages are apparent. D. goodfellowi, D. matschiei and D. spadix form a monophyletic group of closely related taxa, as do the two sampled subspecies of D. dorianus. However, unexpectedly large divergences were obtained both between these subspecies (7.4%) and within one of them (D. d. stellerum, 7.8%). This suggests that D. dorianus may actually consist of a complex of related species, but more extensive research is required.

Avise, J.C., Walker, D., and Johns, G.C. 1998. Speciation durations and Pleistocene effects on vertebrate phylogeography. Proceedings of the Royal Society of London. Series B: Biological Sciences 265: 1707-1712.
,
Speciation durations and Pleistocene effects on vertebrate phylogeography
,
Proceedings of the Royal Society of London. Series B: Biological Sciences
, vol.
265
(pg.
1707
-
1712
)
Bowyer, J.C., Newell, G.R., and Eldridge, M.D.B. 2002. Genetic effects of habitat contraction on Lumholtz's treekangaroo (Dendrolagus lumholtzi) in the Australian Wet Tropics. Conservation Genetics 3: 61-69.
,
Genetic effects of habitat contraction on Lumholtz's treekangaroo (Dendrolagus lumholtzi) in the Australian Wet Tropics
,
Conservation Genetics
, vol.
3
(pg.
61
-
69
)
Brown, W.M., George, M., and Wilson, A.C. 1979. Rapid evolution of animal mitochondrial DNA. Proceedings of the National Academy of Sciences, USA 76: 1967-1971.
,
Rapid evolution of animal mitochondrial DNA
,
Proceedings of the National Academy of Sciences, USA
, vol.
76
(pg.
1967
-
1971
)
Campeau-Peloquin, A., Kirsch, J.A.W., Eldridge, M.D.B., and Lapointe, F.J. 2001. Phylogeny of the rock-wallabies, Petrogale (Marsupialia: Macropodidae) based on DNA/DNA hybridisation. Australian Journal of Zoology 49: 463-486.
,
Phylogeny of the rock-wallabies, Petrogale (Marsupialia: Macropodidae) based on DNA/DNA hybridisation
,
Australian Journal of Zoology
, vol.
49
(pg.
463
-
486
)
Cunningham, M., and Moritz, C. 1998. Genetic effects of forest fragmentation on a rainforest lizard (Scincidae: Gnypetoscincus queenslandiae). Biological Conservation 83: 19-30.
,
Genetic effects of forest fragmentation on a rainforest lizard (Scincidae: Gnypetoscincus queenslandiae)
,
Biological Conservation
, vol.
83
(pg.
19
-
30
)
Felsenstein, J. 1983. Parsimony in systematics: Biological and statistical issues. Annual Review of Ecology and Systematics 14: 313-333.
,
Parsimony in systematics: Biological and statistical issues
,
Annual Review of Ecology and Systematics
, vol.
14
(pg.
313
-
333
)
Felsenstein, J. 1985. Confidence limits on phylogenies: An approach using the bootstrap. Evolution 39: 783-791.
,
Confidence limits on phylogenies: An approach using the bootstrap
,
Evolution
, vol.
39
(pg.
783
-
791
)
Flannery, T.F., Boeadi, and Szalay, A.L. 1995. A new treekangaroo (Dendrolagus, Marsupialia) from Irian Jaya, Indonesia, with notes on ethnography and the evolution of tree-kangaroos. Mammalia 59: 65-84.
,
A new treekangaroo (Dendrolagus, Marsupialia) from Irian Jaya, Indonesia, with notes on ethnography and the evolution of tree-kangaroos
,
Mammalia
, vol.
59
(pg.
65
-
84
)
Flannery, T.F., Martin, R., and Szalay, A.L. 1996. Treekangaroos. A Curious Natural History. Reed Books, Melbourne.
,
Treekangaroos. A Curious Natural History
Girman, D.J. 1996. The use of PCR-based single-stranded conformation polymorphism (PCR-SSCP) in conservation genetics. Pp. 167-182. in Molecular Genetic Approaches in Conservation, edited by T.B. Smith, and R.K. Wayne. Oxford University Press, New York.
,
Molecular Genetic Approaches in Conservation
(pg.
167
-
182
)
Groves, C.P. 1982. The systematics of tree kangaroo (Dendrolagus; Marsupialia, Macropodida). Australian Mammalogy 5: 157-186.
,
The systematics of tree kangaroo (Dendrolagus; Marsupialia, Macropodida)
,
Australian Mammalogy
, vol.
5
(pg.
157
-
186
)
Irwin, D.M., Kocher, T.D., and Wilson, A.C. 1991. Evolution of the cytochrome b gene of mammals. Journal of Molecular Evolution 32: 128-144.
,
Evolution of the cytochrome b gene of mammals
,
Journal of Molecular Evolution
, vol.
32
(pg.
128
-
144
)
Joseph, L., and Moritz, C. 1993. Phylogeny and historical aspects of the ecology of eastern Australian scrubwrens Sericornis spp. - evidence from mitochondrial DNA. Molecular Ecology 2: 161-170.
,
Phylogeny and historical aspects of the ecology of eastern Australian scrubwrens Sericornis spp. - evidence from mitochondrial DNA
,
Molecular Ecology
, vol.
2
(pg.
161
-
170
)
Joseph, L., and Moritz, C. 1994. Mitochondrial DNA phylogeography of birds in eastern Australian rainforests: First fragments. Australian Journal of Zoology 42: 385-403.
,
Mitochondrial DNA phylogeography of birds in eastern Australian rainforests: First fragments
,
Australian Journal of Zoology
, vol.
42
(pg.
385
-
403
)
Joseph, L., Moritz, C., and Hugall, A. 1995. Molecular support for vicariance as a source of diversity in rainforest. Proceedings of the Royal Society of London. Series B: Biological Sciences 260: 177-182.
,
Molecular support for vicariance as a source of diversity in rainforest
,
Proceedings of the Royal Society of London. Series B: Biological Sciences
, vol.
260
(pg.
177
-
182
)
Kawei, M.H. 1989. Geographic variation in the tree kangaroo Dendrolagus dorianus (Marsupialia; Macropodidae). Science in New Guinea 15: 85-94.
,
Geographic variation in the tree kangaroo Dendrolagus dorianus (Marsupialia; Macropodidae)
,
Science in New Guinea
, vol.
15
(pg.
85
-
94
)
Kimura, M. 1980. A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences. Journal of Molecular Evolution 16: 111-120.
,
A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences
,
Journal of Molecular Evolution
, vol.
16
(pg.
111
-
120
)
Kirsch, J.A.W., Lapointe, F.J., and Springer, M.S. 1997. DNAhybridisation studies of marsupials and their implications for metatherian classification. Australian Journal of Zoology 45: 211-280.
,
DNAhybridisation studies of marsupials and their implications for metatherian classification
,
Australian Journal of Zoology
, vol.
45
(pg.
211
-
280
)
Krajewski, C., Blacket, M.J., Buckley, L., and Westerman, M. 1997. A multigene assessment of phylogentic relationships within the dasyurid marsupial subfamily Sminthopsinae. Molecular Phylogenetics and Evolution 8: 236-248.
,
A multigene assessment of phylogentic relationships within the dasyurid marsupial subfamily Sminthopsinae
,
Molecular Phylogenetics and Evolution
, vol.
8
(pg.
236
-
248
)
Martin, R. 1992. An ecological study of Bennett's tree kangaroo (Dendrolagus bennettianus). Report to World Wide Fund for Nature, Sydney.
Martin, R. 1995. Field observations of predation on Bennett's tree kangaroo (Dendrolagus bennettianus) by an amethystine python (Morelia amethistina). Herpetological Review 26: 74-76.
,
Field observations of predation on Bennett's tree kangaroo (Dendrolagus bennettianus) by an amethystine python (Morelia amethistina)
,
Herpetological Review
, vol.
26
(pg.
74
-
76
)
Moritz, C., Patton, J.L., Schneider, C.J., and Smith, T.B. 2000. Diversification of rainforest faunas: An integrated molecular approach. Annual Review of Ecology and Systematics 31: 533-563.
,
Diversification of rainforest faunas: An integrated molecular approach
,
Annual Review of Ecology and Systematics
, vol.
31
(pg.
533
-
563
)
Newell, G.R. 1999a. Responses of Lumholtz's tree-kangaroo (Dendrolagus lumholtzi) to loss of habitat within a tropical rainforest fragment. Biological Conservation 91: 181-189.
,
Responses of Lumholtz's tree-kangaroo (Dendrolagus lumholtzi) to loss of habitat within a tropical rainforest fragment
,
Biological Conservation
, vol.
91
(pg.
181
-
189
)
Newell, G.R. 1999b. Home range and habitat use by Lumholtz's tree-kangaroo (Dendrolagus lumholtzi) within a rainforest fragment in north Queensland. Wildlife Research 26: 129-145.
,
Home range and habitat use by Lumholtz's tree-kangaroo (Dendrolagus lumholtzi) within a rainforest fragment in north Queensland
,
Wildlife Research
, vol.
26
(pg.
129
-
145
)
Newell, G.R. 1999c. Australia's tree-kangaroos: current issues in their conservation. Biological Conservation 87: 1-12.
,
Australia's tree-kangaroos: current issues in their conservation
,
Biological Conservation
, vol.
87
(pg.
1
-
12
)
Osborne, M.J., Norman, J.A., Christidis, L., and Murray, N.D. 2000. Genetic distinctness of isolated populations of an endangered marsupial, the mountain pygmy-possum, Burramys parvus. Molecular Ecology 9: 609-613.
,
Genetic distinctness of isolated populations of an endangered marsupial, the mountain pygmy-possum, Burramys parvus
,
Molecular Ecology
, vol.
9
(pg.
609
-
613
)
Pope, L.C., Estoup, A., and Moritz, C. 2000. Phylogeography and population structure of an ectonal marsupial, Bettongia tropica, determined from mtDNA and microsatellites. Molecular Ecology 9: 2041-2053.
,
Phylogeography and population structure of an ectonal marsupial, Bettongia tropica, determined from mtDNA and microsatellites
,
Molecular Ecology
, vol.
9
(pg.
2041
-
2053
)
Procter-Gray, E. 1984. Dietry ecology of the coppery brushtail possum, green ringtail possum and Lumholtz's tree-kangaroo in north Queensland. Pp. 129-135. in Possums and Gliders, edited by A.P. Smith, and I.D. Hume. Australian Mammal Society, Sydney.
,
Possums and Gliders
(pg.
129
-
135
)
Procter-Gray, E. 1985. The behaviour and ecology of Lumholtz's tree-kangaroo, Dendrolagus lumholtzi (Marsupialia: Macropodidae). Ph.D. thesis, Harvard University,
Rothschild, W., and Dollman, G. 1936. The genus Dendrolagus. Transactions of the Zoological Society of London 21: 477-548.
,
The genus Dendrolagus
,
Transactions of the Zoological Society of London
, vol.
21
(pg.
477
-
548
)
Saitou, N., and Nei, M. 1987. The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution 4: 406-425.
,
The neighbor-joining method: A new method for reconstructing phylogenetic trees
,
Molecular Biology and Evolution
, vol.
4
(pg.
406
-
425
)
Schneider, C.J., and Moritz, C. 1999. Rainforest refugia and evolution in Australia's Wet Tropics. Proceedings of the Royal Society of London. Series B: Biological Sciences 266: 191-196.
,
Rainforest refugia and evolution in Australia's Wet Tropics
,
Proceedings of the Royal Society of London. Series B: Biological Sciences
, vol.
266
(pg.
191
-
196
)
Schneider, C.J., Cunningham, M., and Moritz, C. 1998. Comparative phylogeography and the history of endemic vertebrates in the Wet Tropics rainforests of Australia. Molecular Ecology 7: 487-498.
,
Comparative phylogeography and the history of endemic vertebrates in the Wet Tropics rainforests of Australia
,
Molecular Ecology
, vol.
7
(pg.
487
-
498
)
Schneider, C.J., Smith, T.B., Larison, B., and Moritz, C. 1999. A test of alternative models of diversification in tropical rainforests: Ecological gradients vs. rainforest refugia. Proceedings of the National Academy of Sciences, USA 96: 13869-13873.
,
A test of alternative models of diversification in tropical rainforests: Ecological gradients vs. rainforest refugia
,
Proceedings of the National Academy of Sciences, USA
, vol.
96
(pg.
13869
-
13873
)
Sinclair, E.A., and Westerman, M. 1997. Phylogenetic relationships within the genus Potorous (Marsupialia: Potoroidae) based on allozyme electrophoresis and sequence analysis of the cytochrome b gene. Journal of Mammalian Evolution 4: 147-161.
,
Phylogenetic relationships within the genus Potorous (Marsupialia: Potoroidae) based on allozyme electrophoresis and sequence analysis of the cytochrome b gene
,
Journal of Mammalian Evolution
, vol.
4
(pg.
147
-
161
)
Sunnucks, P., and Hales, D.F. 1996. Numerous transposed sequences of mitochondrial cytochrome oxidase I-II in aphids of the genus Sitobion (Hemiptera: Aphididae). Molecular Biology and Evolution 13: 510-524.
,
Numerous transposed sequences of mitochondrial cytochrome oxidase I-II in aphids of the genus Sitobion (Hemiptera: Aphididae)
,
Molecular Biology and Evolution
, vol.
13
(pg.
510
-
524
)
Sunnucks, P., Wilson, A.C., Beheregaray, L.B., Zenger, K., French, J., and Taylor, A.C. 2000. SSCP is not so difficult: the application and utility of single-stranded conformation polymorphism in evolutionary biology and molecular ecology. Molecular Ecology 9: 1699-1710.
,
SSCP is not so difficult: the application and utility of single-stranded conformation polymorphism in evolutionary biology and molecular ecology
,
Molecular Ecology
, vol.
9
(pg.
1699
-
1710
)
Swofford, D.L. 2000. PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4.0 (beta 8). Sinauer Associates Inc, Sunderland, Massachusetts.
Tamura, K., and Nei, M. 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10: 512-526.
,
Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees
,
Molecular Biology and Evolution
, vol.
10
(pg.
512
-
526
)
Tate, G.H.H. 1948. Results of the Archbold Expeditions. No. 59. Studies on the anatomy and phylogeny of the Macropodidae (Marsupialia). Bulletin of the American Museum of Natural History 91: 239-351.
,
Results of the Archbold Expeditions. No. 59. Studies on the anatomy and phylogeny of the Macropodidae (Marsupialia)
,
Bulletin of the American Museum of Natural History
, vol.
91
(pg.
239
-
351
)
Taylor, A.C., Sherwin, W.B., and Wayne, R.K. 1994. Genetic variation of microsatellite loci in a bottlenecked species: the northern hairy-nosed wombat Lasiorhinus krefftii. Molecular Ecology 3: 277-290.
,
Genetic variation of microsatellite loci in a bottlenecked species: the northern hairy-nosed wombat Lasiorhinus krefftii
,
Molecular Ecology
, vol.
3
(pg.
277
-
290
)
Taylor, A.C., Sunnucks, P., and Cooper, D.W. 1999. Retention of reproductive barriers and ecological differences between two introduced sympatric Macropus spp. in New Zealand. Animal Conservation 2: 195-202.
,
Retention of reproductive barriers and ecological differences between two introduced sympatric Macropus spp. in New Zealand
,
Animal Conservation
, vol.
2
(pg.
195
-
202
)
Winter, J.W. 1997. Responses of non-volant mammals to Late Quaternary climatic changes in the Wet Tropics region of northeastern Australia. Wildlife Research 24: 493-511.
,
Responses of non-volant mammals to Late Quaternary climatic changes in the Wet Tropics region of northeastern Australia
,
Wildlife Research
, vol.
24
(pg.
493
-
511
)
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