The acidification of oceans is predicted to fundamentally alter marine ecosystems. Previous studies have found that elevated CO2 has an effect on adult calcification, fertilisation and larval development,perhaps because of the organisms' inability to regulate acid-base status, but little is known about the mechanisms that underlie such responses. This study investigated the growth response of larvae of a wild and selectively bred line of the Sydney rock oyster, Saccostrea glomerata, to elevated CO2 and measured the pattern of expression of proteins. Overall exposure to elevated CO2 caused a significant reduction in the shell length of D-veliger larvae of the wild, but not in the selectively bred line. Prior to this study, differences in growth between selectively bred and wild oysters have only been found following settlement. Proteome analysis of D-veliger larvae using two-dimensional gel electrophoresis detected a significantly greater number of protein spots in selectively bred compared to wild oyster lines. In addition, a comparison of the proteins expressed between selectively bred and wild larvae exposed to elevated CO2 and ambient conditions showed that a number of proteins were up- or down-regulated and in some cases, switched on at elevated CO2 in selectively bred lines, but not found in the wild lines. Identification of these differentially expressed proteins may assist to "climate proof"of important aquacultural industries.
Bennett, C.A., Franklin, N.L. and 1954. Statistical analysis in chemistry and the chemical industry. John Wiley and Sons, New York, 319 p.
,
Statistical analysis in chemistry and the chemical industry
pg. 319
Bradley, B.P., Gonzalez, C.M., Bond, J.A. and Tepper, B.E. 1994. Complex mixture analysis using protein expression as a quantitative and qualitative tool. Environmental Toxicology and Chemistry 13: 1043–1050.
,
Complex mixture analysis using protein expression as a quantitative and qualitative tool
, Environmental Toxicology and Chemistry
, vol. 13
(pg. 1043
-1050
) Butler, J.N. 1982. Carbon dioxide equilibria and their applications. Addison-Wesley Publishing Company, 259 p.
,
Carbon dioxide equilibria and their applications
pg. 259
Butt, D., Shaddick, K. and Raftos, D. 2006. The effect of low salinity on phenoloxidase activity in the Sydney rock oyster, Saccostrea glomerata. Aquaculture 251: 159–166.
,
The effect of low salinity on phenoloxidase activity in the Sydney rock oyster, Saccostrea glomerata
, Aquaculture
, vol. 251
(pg. 159
-166
) Cameron, J.N. 1986. Acid-base equilibria in invertebrates. In: Heisler N (Ed) Acid-base regulation in animals. Elsevier, New York, p 357–394.
(pg.
357
-394
) Cao, A., Fuented, J., Comesaña, P., Casas, S.M. and Villalba, A. 2009 A proteomic approach envisaged to analyse the bases of oyster tolerance/resistance to bonamiosis. Aquaculture 295: 149–156.
,
A proteomic approach envisaged to analyse the bases of oyster tolerance/resistance to bonamiosis
, Aquaculture
, vol. 295
(pg. 149
-156
) Comeau, S., Gorsky, G., Alliouane, S. and Gattuso J-P. 2010 Larvae of the pteropod Cavolinia inflexa exposed to aragonite undersaturation are viable but shell-less. Marine Biology 157: 2341-2345.
,
Larvae of the pteropod Cavolinia inflexa exposed to aragonite undersaturation are viable but shell-less
, Marine Biology
, vol. 157
(pg. 2341
-2345
) Damiens, G., Mouneyrac, C., Quiniou, F., His, E., Gnassia-Barelli, M. and Roméo, M. 2004. Metal bioaccumulation and metallothionein concentrations in larvae of Crassostrea gigas. Environmental Pollution 140: 492–499.
,
Metal bioaccumulation and metallothionein concentrations in larvae of
, Crassostrea gigas. Environmental Pollution
, vol. 140
(pg. 492
-499
) deBoer, M.L., Krupp, D.A. and Weis, V.M. 2007. Proteomic and transcriptional analyses of coral larvae newly engaged in symbiosis with dinoflagellates. Comparative Biochemistry and Physiology - Part D 2: 63–73.
,
Proteomic and transcriptional analyses of coral larvae newly engaged in symbiosis with dinoflagellates
, Comparative Biochemistry and Physiology - Part D
, vol. 2
(pg. 63
-73
) Dheilly, N.M., Nair, S.V., Smith, L.C. and Raftos, D.A. 2009. Highly variable immune-response proteins (185/333) from the sea urchin, Strongylocentrotus purpuratus: proteomic analysis identifies diversity within and between individuals. Journal of Immunology 182 (4): 2203–2212.
,
Highly variable immune-response proteins (185/333) from the sea urchin, Strongylocentrotus purpuratus: proteomic analysis identifies diversity within and between individuals
, Journal of Immunology
, vol. 182
(pg. 2203
-2212
) Dupont, S., Wilson, K., Obst, M., Sköld, H., Nakano, H. and Thorndyke, M.C. 2007. Marine ecological genomics: when genomics meets marine ecology. Marine Ecology Progress Series 332: 257–273.
,
Marine ecological genomics: when genomics meets marine ecology
, Marine Ecology Progress Series
, vol. 332
(pg. 257
-273
) Ellis, R.P., Bersey, J., Rundle, S.D., Hall-Spencer, J.M. and Spicer, J.I. 2009. Subtle but significant effects of CO2 acidified seawater on embryos of the intertidal snail, Littorina obtusata. Aquatic Biology 5: 41-48.
,
Subtle but significant effects of CO2 acidified seawater on embryos of the intertidal snail, Littorina obtusata
, Aquatic Biology
, vol. 5
(pg. 41
-48
) Fabry, V.J., Seibel, B.A., Feely, R.A., Orr, J.C. 2008. Impacts of ocean acidification on marine fauna and ecosystem processes. International Council for the Exploration of the Sea (ICES) 65: 414–432.
,
Impacts of ocean acidification on marine fauna and ecosystem processes
, International Council for the Exploration of the Sea (ICES)
, vol. 65
(pg. 414
-432
) Gran, G. 1952. Determination of the equivalence point in potentiometric titrations – Part II. The Analyst 77: 661–671.
,
Determination of the equivalence point in potentiometric titrations – Part II
, The Analyst
, vol. 77
(pg. 661
-671
) Gutowska, M.A., Pörtner HO. and Melzner, F. 2008. Growth and calcification in the cephalopod Sepia officinalis under elevated seawater pCO2. Marine Ecology Progress Series 373: 303-309.
,
Growth and calcification in the cephalopod Sepia officinalis under elevated seawater pCO2
, Marine Ecology Progress Series
, vol. 373
(pg. 303
-309
) Houghton, J.T., Filho, L.G.M., Callander, B.H., Harris, N., Kattenberg, A. and Maskell, K. 1996. Climate Change 1995. The science of climate change. Contribution of Working Group II to the Second Assessment Report of the Intergovernmental Panel on Climate Change (IPCC). Cambridge University Press, New York, p 19–24.
,
Climate Change 1995. The science of climate change. Contribution of Working Group II to the Second Assessment Report of the Intergovernmental Panel on Climate Change (IPCC)
(pg. 19
-24
) Houghton, J.T., Ding, Y., Griggs, D.J., Noguer, M., van der Linden, P.J., Dai, X., Maskell, K. and Johnson, C.A. 2001. Climate change 2001: The Scientific Basis. Contribution of Working Group I to the Third Assessment Report of the Intergovernmental Panel on Climate Change (IPCC). Cambridge University Press, New York, USA, p 1–83.
,
Climate change 2001: The Scientific Basis. Contribution of Working Group I to the Third Assessment Report of the Intergovernmental Panel on Climate Change (IPCC)
(pg. 1
-83
) Jellum, E., Thorsrud, A.K. and Karasek, F.W. 1983. Two-dimensional electrophoresis for determining toxicity of environmental substances. Analytical Chemistry 55: 2340–2344.
,
Two-dimensional electrophoresis for determining toxicity of environmental substances
, Analytical Chemistry
, vol. 55
(pg. 2340
-2344
) Keppler, C. and Ringwood, A.H. 2001. Expression of P-glycoprotein in the gills of oysters, Crassostrea virginica: seasonal and pollutant related effects. Aquatic Toxicology 54 (3–4): 195–204.
,
Expression of P-glycoprotein in the gills of oysters, Crassostrea virginica: seasonal and pollutant related effects
, Aquatic Toxicology
, vol. 54
(pg. 3, 195
-4, 204
) Kochevar, R.E. and Childress, J.J. 1996. Carbonic anhydrase in deep-sea chemoautotrophic symbiosis. Marine Biology 125: 375-383.
,
Carbonic anhydrase in deep-sea chemoautotrophic symbiosis
, Marine Biology
, vol. 125
(pg. 375
-383
) Kurihara, H., Kato, S. and Ishimatsu, A. 2007. Effects of increased seawater pCO on early development of the oyster Crassostrea gigas. Aquatic Biology 1: 91-98.
,
Effects of increased seawater pCO on early development of the oyster Crassostrea gigas
, Aquatic Biology
, vol. 1
(pg. 91
-98
) Kurihara, H., Asai, T., Kato, S. and Ishimatsu, A. 2008 Effects of elevated pCO2 on early development in the mussel Mytilus galloprovincialis. Aquatic Biology 4: 225-233.
,
Effects of elevated pCO2 on early development in the mussel
, Mytilus galloprovincialis. Aquatic Biology
, vol. 4
(pg. 225
-233
) López, J.L. 2007. Applications of proteomics in marine ecology. Marine Ecology Progress Series 332: 275–279.
,
Applications of proteomics in marine ecology
, Marine Ecology Progress Series
, vol. 332
(pg. 275
-279
) López, J.L., Lorenzo, S. and Fuentes, J. 2005. Proteomic approach to probe for larval proteins of the mussel Mytilus galloprovincialis. Marine Biotechnology 7: 396–404.
,
Proteomic approach to probe for larval proteins of the mussel
, Mytilus galloprovincialis. Marine Biotechnology
, vol. 7
(pg. 396
-404
) Medakovic, D. 2000. Carbonic anhydrase activity and biomineralization process in embryos, larvae and adult blue mussels Mytilus edulis L. Helgoland Marine Research 54: 1–6.
,
Carbonic anhydrase activity and biomineralization process in embryos, larvae and adult blue mussels Mytilus edulis L
, Helgoland Marine Research
(pg. 1
-6
) Medakovic, D. and Lucu, C. 1994. Distribution of carbonic anhydrase in larval and adult mussels Mytilus edulis Linnaeus. Periodicum Biologorum 96: 452–454.
,
Distribution of carbonic anhydrase in larval and adult mussels Mytilus edulis Linnaeus
, Periodicum Biologorum
, vol. 96
(pg. 452
-454
) Medeiros, I.D., Siebert, M.N., de Toledo e Silva, G., Moraes, M.O., Marques, M.R., Bainy, A.C. 2008. Differential gene expression in oyster exposed to sewage. Marine Environmental Research 66 (1): 156–157.
,
Differential gene expression in oyster exposed to sewage
, Marine Environmental Research
, vol. 66
(pg. 156
-157
) Melzner, F., Göbel, S., Langenbuch, M., Gutowska, M.A., Pörtner, HO., Lucassen, M. 2009. Swimming performance in Atlantic Cod (Gadus morhua) following long-term (4-12 months) acclimation to elevated seawater pCO2. Aquatic Toxicology 92: 30-37.
,
Swimming performance in Atlantic Cod (Gadus morhua) following long-term (4-12 months) acclimation to elevated seawater pCO2
, Aquatic Toxicology
, vol. 92
(pg. 30
-37
) Metzger, R., Sartoris, F.J., Langenbuch, M. and Pörtner, HO. 2007. Influence of elevated CO2 concentrations on thermal tolerance of the edible crab Cancer pagurus. Journal of Thermal Biology 32: 144-151.
,
Influence of elevated CO2 concentrations on thermal tolerance of the edible crab Cancer pagurus
, Journal of Thermal Biology
, vol. 32
(pg. 144
-151
) Michaelidis, B., Ouzounts, C., Paleras, A. and Pörtner, HO. 2005. Effects of long-term moderate hypercapnia on acid–base balance and growth rate in marine mussels Mytilus galloprovincialis. Marine Ecology Progress Series 293: 109-118.
,
Effects of long-term moderate hypercapnia on acid–base balance and growth rate in marine mussels Mytilus galloprovincialis
, Marine Ecology Progress Series
, vol. 293
(pg. 109
-118
) Miles, H., Widdicombe, S., Spicer, J.I. and Hall-Spencer, J. 2007. Effects of anthropogenic seawater acidification on acidbase balance in the sea urchin Psammechinus miliaris. Marine Pollution Bulletin 54: 89–96.
,
Effects of anthropogenic seawater acidification on acidbase balance in the sea urchin
, Psammechinus miliaris. Marine Pollution Bulletin
, vol. 54
(pg. 89
-96
) Mok, F.S.Y., Thiyagarajan, V. and Qian, P.Y. 2009. Proteomic analysis during larval development and metamorphosis of the spionid polychaete Pseudopolydora vexillosa. Proteome Science 7 (44): doi:10.1186/1477-5956-7-44.
,
Proteomic analysis during larval development and metamorphosis of the spionid polychaete
, Pseudopolydora vexillosa. Proteome Science
, vol. 7
Newton, K., Peters, R. and Raftos, D. 2004. Phenoloxidase and QX disease resistance in Sydney rock oysters (Saccostrea glomerata). Developmental and Comparative Immunology 28: 565–569.
,
Phenoloxidase and QX disease resistance in Sydney rock oysters (Saccostrea glomerata)
, Developmental and Comparative Immunology
, vol. 28
(pg. 565
-569
) O'Connor, W.A., Dove, M.C., Finn, B. and O'Connor, S.J. 2008. Manual for hatchery production of Sydney rock oysters (Saccostrea glomerata). Final report to Fisheries Research and Development Corporation, Deakin, ACT, Australia. New South Wales Department of Primary Industries – Fisheries Research Report Series, 20: 55 p.
Parker, L.M., Ross, P.M. and O'Connor, W.A. 2009. The effect of ocean acidification and temperature on the fertilization and embryonic development of the Sydney rock oyster Saccostrea glomerata (Gould 1850). Global Change Biology 15: 2123–2136.
,
The effect of ocean acidification and temperature on the fertilization and embryonic development of the Sydney rock oyster Saccostrea glomerata (Gould 1850)
, Global Change Biology
, vol. 15
(pg. 2123
-2136
) Parker, L.M., Ross, P.M. and O'Connor W.A. 2010. Comparing the effect of elevated pCO2 and temperature on the reproduction and early development of two species of oysters. Marine Biology 157 (11): 2435-2452.
,
Comparing the effect of elevated pCO2 and temperature on the reproduction and early development of two species of oysters
, Marine Biology
, vol. 157
(pg. 2435
-2452
) Parker, L.M., Ross, P.M. and O'Connor, W.A. 2011. Populations of the Sydney rock oysters, Saccostrea glomerata vary in response to ocean acidification. Marine Biology 158: 689-697.
,
Populations of the Sydney rock oysters, Saccostrea glomerata vary in response to ocean acidification
, Marine Biology
, vol. 158
(pg. 689
-697
) Peters, R. and Raftos, D.A. 2003. The role of phenoloxidase suppression in QX disease outbreaks among Sydney rock oysters (Saccostrea glomerata). Aquaculture 223: 29–39.
,
The role of phenoloxidase suppression in QX disease outbreaks among Sydney rock oysters (Saccostrea glomerata)
, Aquaculture
, vol. 223
(pg. 29
-39
) Piano, A., Valbonesi, P. and Fabbri, E. 2004. Expression of cytoprotective proteins, heat shock protein 70 and metallothioneins, in tissue of Ostrea edulis exposed to heat and heavy metals. Cell Stress Chaperones 9 (2): 134–142.
,
Expression of cytoprotective proteins, heat shock protein 70 and metallothioneins, in tissue of Ostrea edulis exposed to heat and heavy metals
, Cell Stress Chaperones
, vol. 9
(pg. 134
-142
) Picchietti, S., Fausto, A.M., Randelli, E., Carnevali, O., Taddei, A.R., Buonocore, F., Scapigliati, G. and Abelli, L. 2009. Early treatment with Lactobacillus delbrueckii strain induces an increase in intestinal T-cells and granulocytes and modulates immune-related genes of larval Dicentrarchus labrax (L.). Fish and Shellfish Immunology 26: 368–376.
,
Early treatment with Lactobacillus delbrueckii strain induces an increase in intestinal T-cells and granulocytes and modulates immune-related genes of larval Dicentrarchus labrax (L.)
, Fish and Shellfish Immunology
, vol. 26
(pg. 368
-376
) Pörtner, HO. 2008. Ecosystem effects of ocean acidification in times of ocean warming: a physiologist's view. Marine Ecology Progress Series 373: 203–217.
,
Ecosystem effects of ocean acidification in times of ocean warming: a physiologist's view
, Marine Ecology Progress Series
, vol. 373
(pg. 203
-217
) Pörtner, HO., Reipschläger, A. and Heisler, N. 1998. Acidbase regulation, metabolism and energetics in Sipunculus nudus as a function of ambient carbon dioxide level. Journal of Experimental Biology 201: 43–54.
,
Acidbase regulation, metabolism and energetics in Sipunculus nudus as a function of ambient carbon dioxide level
, Journal of Experimental Biology
, vol. 201
(pg. 43
-54
) Reipschläger, A., Pörtner, HO. 1996. Metabolic depression during environmental stress: the role of extracellular versus intracellular pH in Sipunculus nudus. The Journal of Experimental Biology 199: 1801–1807.
,
Metabolic depression during environmental stress: the role of extracellular versus intracellular pH in
, Sipunculus nudus. The Journal of Experimental Biology
, vol. 199
(pg. 1801
-1807
) Shepard, J.L. and Bradley, B.P. 2000. Protein expression signatures and lysosomal stability in Mytilus edulis exposed to graded copper concentrations. Marine Environmental Research 50: 457–463.
,
Protein expression signatures and lysosomal stability in Mytilus edulis exposed to graded copper concentrations
, Marine Environmental Research
, vol. 50
(pg. 457
-463
) Simonian, M., Nair, S.V., O'Connor, W.A. and Raftos, D.A. 2009a. Protein markers of Marteilia sydneyi infection in Sydney rock oysters, Saccostrea glomerata. Journal of Fish Diseases 32: 367–375.
,
Protein markers of Marteilia sydneyi infection in Sydney rock oysters
, Saccostrea glomerata. Journal of Fish Diseases
, vol. 32
(pg. 367
-375
) Simonian, M., Nair, S.V., Nell, J.A., Raftos, D.A. 2009b. Proteomic clues to the identification of QX disease-resistant biomarkers in selectively bred Sydney rock oysters, Saccostrea glomerata. Journal of Proteomics 73 (2): 209–217.
,
Proteomic clues to the identification of QX disease-resistant biomarkers in selectively bred Sydney rock oysters, Saccostrea glomerata
, Journal of Proteomics
, vol. 73
(pg. 209
-217
) Sokal, R.R. and Rohlf, F.J. 1995. Biometry: The principles and practice of statistics in biological research. WH Freeman and Company, New York, 3rd Edition, 887 p.
,
Biometry: The principles and practice of statistics in biological research
pg. 887
Solomon, S., Qin, D., Manning, M., Alley, R.B., Berntsen, T., Bindoff, N.L., Chen, Z., Chidthaisong, A., Gregory, J.M., Hegerl, G.C., Heimann, M., Hewitson, B., Hoskin, B.J., Joos, F., Jouzel, J., Kattsov, V., Lohmann, U., Matsuno, T., Molina, M., Nicholls, N., Overpeck, J., Raga, G., Ramaswamy, V., Ren, J., Rusticucci, M., Somerville, R., Stocker, TF., Whetton, P., Wood, RA. and Wratt, D. 2007. Climate Change 2007: The Physical Science Basis. Contribution of Working Group 1 to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change (IPCC). Cambridge University Press, Cambridge UK, and New York, USA.
,
The Physical Science Basis. Contribution of Working Group 1 to the Fourth Assessment Report of the Intergovernmental Panel on Climate Change (IPCC)
Spicer JI, Raffo A, Widdicombe S (2007) Influence of CO2- related seawater acidification on extracellular acid–base balance in the velvet swimming crab Necora puber. Marine Biology 151: 1117–1125.
,
Influence of CO2- related seawater acidification on extracellular acid–base balance in the velvet swimming crab
, Necora puber. Marine Biology
, vol. 151
(pg. 1117
-1125
) Stumpp, M., Dupont, S., Thorndyke M.C. and Melzner F. 2011. CO2 induced seawater acdificiation impacts sea urchin larval development II: Gene expression patterns in pluteus larvae. Comparative Biochemistry and Physiology Part A 160: 320-330.
,
CO2 induced seawater acdificiation impacts sea urchin larval development II: Gene expression patterns in pluteus larvae
, Comparative Biochemistry and Physiology Part A
, vol. 160
(pg. 320
-330
) Sveinsdóttir, H., Steinarsson, A. and Gudmundsdóttir. 2009. Differential protein expression in early Atlantic cod larvae (Gadus morhua) in response to treatment with probiotic bacteria. Comparative Biochemistry and Physiology Part D 4: 249–254.
,
Differential protein expression in early Atlantic cod larvae (Gadus morhua) in response to treatment with probiotic bacteria
, Comparative Biochemistry and Physiology Part D
, vol. 4
(pg. 249
-254
) Tanguy. A., Mura, C. and Moraga, D. 2001. Cloning of a metallothionein gene and characterization of two other cDNA sequences in the Pacific oyster Crassostrea gigas (CgMT1). Aquatic Toxicology 55 (1–2): 35–47.
,
Cloning of a metallothionein gene and characterization of two other cDNA sequences in the Pacific oyster Crassostrea gigas (CgMT1)
, Aquatic Toxicology
, vol. 55
(pg. 1, 35
-2, 47
) Thiyagarajan, V. and Qian, P.Y. 2008. Proteomic analysis of larvae during development, settlement, and metamorphosis in the fouling barnacle, Balanus amphitrite. Proteomics 8: 3164-3172.
,
Proteomic analysis of larvae during development, settlement, and metamorphosis in the fouling barnacle, Balanus amphitrite
, Proteomics
, vol. 8
(pg. 3164
-3172
) Thiyagarajan, V. Wong, T. and Qian, P.Y. 2009. 2D gelbased proteome and phosphoproteome analysis during larval metamorphosis in two major marine biofouling invertebrates. Journal of Proteome Research 8: 2708–2719.
,
2D gelbased proteome and phosphoproteome analysis during larval metamorphosis in two major marine biofouling invertebrates
, Journal of Proteome Research
, vol. 8
(pg. 2708
-2719
) Todgham, A.E. and Hofmann, G.E. 2009. Transcriptomic response of sea urchin larvae Strongylocentrotus purpuratus to CO2-driven seawater acidification. The Journal of Experimental Biology 212: 2579-2594.
,
Transcriptomic response of sea urchin larvae Strongylocentrotus purpuratus to CO2-driven seawater acidification
, The Journal of Experimental Biology
, vol. 212
(pg. 2579
-2594
) Ueda, N., and Boettcher, A. 2009. Differences in heat shock protein 70 expression during larval and early spat development in the Eastern oyster, Crassostrea virginica (Gmelin, 1791). Cell Stress and Chaperones 14: 439–443.
,
Differences in heat shock protein 70 expression during larval and early spat development in the Eastern oyster, Crassostrea virginica (Gmelin, 1791)
, Cell Stress and Chaperones
, vol. 14
(pg. 439
-443
) Underwood, A.J. 1997. Experiments in Ecology: their logical design and interpretation using analysis of variance. Cambridge University Press, Cambridge, 504 p.
,
Experiments in Ecology: their logical design and interpretation using analysis of variance
pg. 504
Waldbusser, G.G, Bergschneider, H., Green, M.A. 2010. Size-dependent pH effect on calcification in post-larval hard clam Mercenaria spp. Marine Ecology Progress Series 417: 171-182.
,
Size-dependent pH effect on calcification in post-larval hard clam Mercenaria spp
, Marine Ecology Progress Series
, vol. 417
(pg. 171
-182
) Welch, W.J. 1993. How cells respond to stress. Scientific American 268 (5): 56–64.
,
How cells respond to stress
, Scientific American
, vol. 268
(pg. 56
-64
) Wilbur, K.M. and Anderson, N.G. 1950. Carbonic andhydrase and growth in the oyster and busycon. The Biological Bulletin 98: 19–24.
,
Carbonic andhydrase and growth in the oyster and busycon
, The Biological Bulletin
, vol. 98
(pg. 19
-24
) Winer, B.J., Brown, D.R. and Michels, K.M. 1991. Statistical Principles in Experimental Design. McGraw Hill, New York, 3rd edition, 1057 p.
,
Statistical Principles in Experimental Design
pg. 1057
Zhao, X., He, H., Dong, D. and Wnag, J. 2006. Identification of differentially expressed proteins during larval molting of Helicoverpa armigera. Journal of Proteome Research 5 (1): 164–169.
,
Identification of differentially expressed proteins during larval molting of Helicoverpa armigera
, Journal of Proteome Research
, vol. 5
(pg. 164
-169
) Zippay M.L. and Hofmann G.E. 2010. Effect of pH on gene expression and thermal tolerance of early life history stages of the red abalone (Haliotis rufescens). Journal of Shellfish Research 29 (2): 429–439.
,
Effect of pH on gene expression and thermal tolerance of early life history stages of the red abalone (Haliotis rufescens)
, Journal of Shellfish Research
, vol. 29
(pg. 429
-439
)
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