Chlamydia psittaci (C. psittaci), an obligated intracellular gram-negative bacteria, causes an important zoonotic disease in humans namely psittacosis. The objective of this study was to determine the persistent viability of C. psittaci in various temperature conditions. The cloacal swab (CS) samples were collected from feral and racing pigeons for the purpose of finding a C. psittaci field strain. The bacterial isolation showed that 1.3% of feral pigeons were PCR positive while all samples of racing pigeons were PCR negative. Also, bacterial characterization suggested that it belonged to genotype B, which had bacterial titers 3.2 and 3.89 log ELD50/ml, respectively. Then a bacterial persistence test was performed and the results showed that C. psittaci could survive at 56oC for up to 72 h. In conclusions, C. psittaci could be found in feral pigeons in central Thailand. The bacteria can survive in equatorial temperature areas. This study was the first report that C. psittaci could survive and has infectivity after 56oC for 72 h. Therefore, the public health awareness of C. psittaci infection in humans should be concerned.