Clostridium perfringens , a commensal of the intestinal tract of many animal species, has been associated with necrotic enteritis (NE), an economically significant poultry disease. C. perfringens is known to survive in the environment for extended periods of time through the formation of spores. These spores have the potential to be transmitted to subsequent flocks. Persistence of a single C. perfringens strain in a broiler chicken farm environment has however been poorly documented. The aim of this study was to compare multiple isolates of C. perfringens collected over time in a single farm with recurrent episodes of necrotic enteritis. Isolates were recovered from the intestines of chickens affected with NE (2014 and 2016 outbreaks) and from healthy chickens (2017), and from environmental samples (2016). PCR characterization of those isolates showed that all sampling groups contained netB-positive isolates except for the environmental samples. Moreover, results showed that all environmental isolates were positive for the cna adhesin, whereas other groups had lower numbers of cna-positive isolates. Biofilm formation assays showed that most of the isolates were able to form biofilm. Pulsed field gel electrophoresis analyses showed that one clone was present in every sampling group with the exception of the 2014 outbreak. However, one clone found in the latter group was highly similar, having 94% similarity with the persistent C. perfringens clone. This study describes for the first time the persistence of a C. perfringens strain on a broiler chicken house over a three-year period.
Persistence of a Clostridium perfringens strain in a broiler chicken farm over a three-year period
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Audrey Charlebois, Eric Parent, Marie-Pierre LÃ©tourneau-Montminy, Martine Boulianne; Persistence of a Clostridium perfringens strain in a broiler chicken farm over a three-year period. Avian Dis doi: https://doi.org/10.1637/aviandiseases-D-19-00112
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