Mycoplasma gallisepticum (MG) is a major pathogen of the poultry industry throughout the world. MG causes chronic respiratory disease in chickens and infectious sinusitis in turkeys. Despite constantly improving the biosecurity of the poultry industry in Iran, MG infection still occurs and causes significant economic issues. To evaluate genetic variability, ten Iranian MG isolates along with 17 available sequences were characterized using gene-targeted sequencing (GTS) analysis of complete mgc2/pvpA genes. According to the findings, the number of strains typed by this method is 21 different sequence types within the sample set of 27 strains. The discriminatory power of this typing assay was established to be 0.97. Although no insertions and deletions of nucleotides were observed in the mgc2 gene among the Iranian strains, different lengths of pvpA genes with 1086, 1095 and 1101 nucleotides were detected within the direct repeat (DR) 1 and 2. Generally, eight tetrapeptides Pro-Arg-Pro-Met/Gln/Asn were found in the DRs of PvpA. Analysis of the carboxyl end of PvpA proteins exhibited various repeats of prolines. Based on the phylogenetic tree of partial and complete mgc2/pvpA genes, all Iranian MG isolates were clustered into two distinct groups. Since this typing assay could provide a higher discriminatory power than previous reported GTS scheme based on partial mgc2/pvpA genes, these results can be considered as the blueprint for future national control and diagnostic strategies. Furthermore, consistent surveillance with larger dataset will be needed to clarify the epidemiological characteristics of MG outbreaks in different poultry hosts.

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