SUMMARY Avian erythroblastosis (erythroid leukosis) was detected in a 78-day-old Japanese native chicken. At necropsy, the liver was enlarged and diffusely dark red in color. Moderate splenomegaly was observed. Histologically, round-to-polygonal tumor cells were observed only in the blood vessels of the liver and other organs. Immunohistochemistry (IHC) was performed on formalin-fixed paraffin-embedded (FFPE) sections to characterize tumor cells. Tumor cells, as well as normal erythrocytes as positive controls, were consistently positive for IHC using the commercially available anti-human hemoglobin antibody. Exogenous avian leukosis virus (ALV) -B and endogenous ALV-E genes were detected by polymerase chain reaction, although ultrastructural observation revealed no viral particles associated with tumor cells. Results suggest that the commercial anti-human hemoglobin antibody used in the study cross-react to chicken erythrocytes on FFPE sections by IHC, and it also can be used for definitive diagnosis of the spontaneous case of avian erythroblastosis in chickens.
Spontaneous Avian Erythroblastosis in a Chicken Confirmed by Immunohistochemical Detection of Hemoglobin in Tumor Cells
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Seiji Fujiki, Aoi Kurokawa, Yu Yamamoto; Spontaneous Avian Erythroblastosis in a Chicken Confirmed by Immunohistochemical Detection of Hemoglobin in Tumor Cells. Avian Dis 2021; doi: https://doi.org/10.1637/aviandiseases-D-21-00030
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