We investigated the reproductive biology of Podocnemis sextuberculata in the Reserva Biológica do Rio Trombetas, state of Pará, Brazil. Female carapace length was positively correlated with number, average weight, and volume of eggs. Clutch size varied from 8 to 24 eggs, incubation period varied from 48 to 64 days, and hatching occurred about 1 month before the beginning of the rainy season. Local inhabitants regularly use females, nests, and neonates as food sources.

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