Six new species of Neopsis from montane Atlantic forest and Cerrado of Brazil (Minas Gerais) and montane Amazon forest of Peru are described and illustrated. Neopsis amazonica Hamilton is newly recorded from Amazonas State (Brazil) and its male is described and illustrated for the first time. The female terminalia of Neopsis robusta Linnavuori and Lonnia pallida (Linnavuori & DeLong) are also illustrated for the first time. A key to all eleven species of the genus is provided. Tribe Neopsini is newly placed in subfamily Tartessinae, a subfamily previously recorded only from the Australian and Indomalayan regions, based on morphological characters and phylogenetic evidence.