Luminescent pigments can be incorporated into coatings for various applications, including safety signage, that provide illuminated directions when lighting systems fail. The influence of the brightness of a solid wood substrate on the persistence performance of luminescent pigments was analyzed. Three species were tested: maple (Acer pseudoplatanus; a brightly colored wood), steamed beech (Fagus sylvatica; a medium-colored wood), and walnut heartwood (Juglans regia; a dark-colored wood). The results show that the surface color has a major influence on the performance of the pigments, when applied in a clear coating. Other significant factors for the afterglowing effect are the amount of pigment and the time and intensity of light during the charging process.