Background:General anesthesia and surgical stress cause an acute endocrine, metabolic, and immunological inflammatory response in organisms and an increase in neutrophil lymphocyte ratio (NLR) and ischemia modified albumin (IMA) levels. Ozone, other than inhalation administration, reduces the release of antioxidants and some proinflammatory cytokines and has been shown to have an anti-inflammatory effect. Our aim is to research how the NLR and IMA response is affected in rabbits undergoing surgical intervention with general anesthesia given prophylactic with ozone therapy. Methods: Twelve New Zealand rabbits were divided into two groups: GroupO was given 70 µg/ml 10 ml ozone by the rectal route in 6 sessions on alternate days, and GroupC was given air by the rectal route.The rabbits underwent surgical intervention under general anesthesia.Blood samples were taken at basal, preoperative, 30th minute of operation, and 24 hour postoperative times and were examined for hemogram and IMA. Results:In the postoperative 24th hour an increase in NLR was observed in both groups, more clearly in GroupC (p<0.05). In both groups, comparisons within the groups showed a significant increase in NLR only in the postoperative 24th hour compared to other times (p<0.05).When IMA values were compared, differences between the groups were observed between preoperative values and those at the 30th minute of anesthesia and 24 hours postoperative (p<0.05). Conclusions:When General anesthesia and surgical stress response was evaluated using inflammatory parameters of both NLR and IMA, there was significantly less of an increase in levels in rabbits given ozone compared to the control group.