Background: Autologous blood is sometimes collected before hepatectomy. The usefulness of ABD has been reported in several studies. However, the relationship between ABD and CKD has never been studied. Aim: The aim of this study was to clarify the relationship between preoperative autologous blood donation (ABD) and chronic kidney disease (CKD) in terms of anemia. Methods: Between January 2006 and December 2012, 70 patients made two ABDs ≥ 600 mL before hepatectomy. CKD stages were based on estimated glomerular filtration rates. Univariate analyses were performed using the Mann-Whitney U test for continuous variables and the chi-square test for nominal variables. Results: Patients at CKD stages < G3b (n = 60) and ≥ G3b (n = 10) were compared. There were no significant differences in hemoglobin level at the initial hospital visit or total volume of ABD between the two groups. However, hemoglobin levels just before the first ABD, the second ABD, and resection, and just after resection were significantly lower in the CKD ≥ G3b group than those in the < G3b group (p < 0.05). Conclusions: CKD stage contributes to reductions in hemoglobin levels after ABD. We recommend the use of erythropoietin in CKD ≥ G3b cases that include ABDs.