Differences in the cytological interpretation of bronchoalveolar lavage fluid (BALF) after cytospin preparation (CP) or manual smearing of pelleted cells preparation (MSP) were investigated in client-owned dogs and cats with inflammatory or infectious lower respiratory disease. Bronchoalveolar lavage fluid from healthy cats was also examined. With MSP, cell lysis was more frequently observed, and cellular distribution was more heterogeneous throughout the slide. When samples from healthy and diseased animals were considered together, a significantly greater percentage of neutrophils was seen on CP than on MSP slides (P<0.002). Cytospin preparations were considered of better quality in all individual comparisons. Cytospin preparation is advised in the evaluation of BALF with low total cell count. When only MSPs are evaluated, clinicians should be aware that differential neutrophil counts may underestimate the counts found on CP slides.
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November/December 2008
Respiratory Diseases|
November 01 2008
Comparison of Bronchoalveolar Lavage Cytospins and Smears in Dogs and Cats
Sandrine Dehard, DVM;
Sandrine Dehard, DVM
Departments of Clinical Sciences (Dehard, Bernaerts, Peeters, McEntee, Clercx) and Quantitative Genetics (Detilleux), Faculty of Veterinary Medicine, University of Liège, Liège, 4000 Belgium; and the
Department of Pathology and Microbiology (Day), Faculty of Veterinary Medicine, University of Bristol, Langford House, Langford, North Somerset, United Kingdom BS40 5DU
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Frederique Bernaerts, DVM;
Frederique Bernaerts, DVM
Departments of Clinical Sciences (Dehard, Bernaerts, Peeters, McEntee, Clercx) and Quantitative Genetics (Detilleux), Faculty of Veterinary Medicine, University of Liège, Liège, 4000 Belgium; and the
Department of Pathology and Microbiology (Day), Faculty of Veterinary Medicine, University of Bristol, Langford House, Langford, North Somerset, United Kingdom BS40 5DU
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Dominique Peeters, DVM, PhD, Diplomate ECVIM-CA;
Dominique Peeters, DVM, PhD, Diplomate ECVIM-CA
Departments of Clinical Sciences (Dehard, Bernaerts, Peeters, McEntee, Clercx) and Quantitative Genetics (Detilleux), Faculty of Veterinary Medicine, University of Liège, Liège, 4000 Belgium; and the
Department of Pathology and Microbiology (Day), Faculty of Veterinary Medicine, University of Bristol, Langford House, Langford, North Somerset, United Kingdom BS40 5DU
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Johann Detilleux, DVM, PhD;
Johann Detilleux, DVM, PhD
Departments of Clinical Sciences (Dehard, Bernaerts, Peeters, McEntee, Clercx) and Quantitative Genetics (Detilleux), Faculty of Veterinary Medicine, University of Liège, Liège, 4000 Belgium; and the
Department of Pathology and Microbiology (Day), Faculty of Veterinary Medicine, University of Bristol, Langford House, Langford, North Somerset, United Kingdom BS40 5DU
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Kathleen McEntee, DVM, PhD, Diplomate ECVIM-CA (Cardiology);
Kathleen McEntee, DVM, PhD, Diplomate ECVIM-CA (Cardiology)
Departments of Clinical Sciences (Dehard, Bernaerts, Peeters, McEntee, Clercx) and Quantitative Genetics (Detilleux), Faculty of Veterinary Medicine, University of Liège, Liège, 4000 Belgium; and the
Department of Pathology and Microbiology (Day), Faculty of Veterinary Medicine, University of Bristol, Langford House, Langford, North Somerset, United Kingdom BS40 5DU
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Michael J. Day, BSc, BVMS, PhD, Diplomate ECVP;
Michael J. Day, BSc, BVMS, PhD, Diplomate ECVP
Departments of Clinical Sciences (Dehard, Bernaerts, Peeters, McEntee, Clercx) and Quantitative Genetics (Detilleux), Faculty of Veterinary Medicine, University of Liège, Liège, 4000 Belgium; and the
Department of Pathology and Microbiology (Day), Faculty of Veterinary Medicine, University of Bristol, Langford House, Langford, North Somerset, United Kingdom BS40 5DU
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Cecile Clercx, DVM, PhD, Diplomate ECVIM-CA
Cecile Clercx, DVM, PhD, Diplomate ECVIM-CA
Departments of Clinical Sciences (Dehard, Bernaerts, Peeters, McEntee, Clercx) and Quantitative Genetics (Detilleux), Faculty of Veterinary Medicine, University of Liège, Liège, 4000 Belgium; and the
Department of Pathology and Microbiology (Day), Faculty of Veterinary Medicine, University of Bristol, Langford House, Langford, North Somerset, United Kingdom BS40 5DU
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J Am Anim Hosp Assoc (2008) 44 (6): 285–294.
Citation
Sandrine Dehard, Frederique Bernaerts, Dominique Peeters, Johann Detilleux, Kathleen McEntee, Michael J. Day, Cecile Clercx; Comparison of Bronchoalveolar Lavage Cytospins and Smears in Dogs and Cats. J Am Anim Hosp Assoc 1 November 2008; 44 (6): 285–294. doi: https://doi.org/10.5326/0440285
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