Hamstring strain injuries are common among athletes and often require rehabilitation to prepare for timely return to sport performance, while also minimizing re-injury risk. Return to sport is typically achieved within weeks of the injury, but subsequent athlete performance may be impaired and re-injury rates are high. Improving these outcomes requires rehabilitation practitioners (e.g. Athletic Trainers and Physical Therapists) to understand the etiology and mechanisms of hamstring strain injury; know how to perform a thorough clinical examination; and progress loading to the site of injury in a safe and effective manner. This narrative review discusses current clinical concepts related to these aspects of hamstring strain injury rehabilitation, with the aim of helping practitioners improve athlete outcomes. Collectively, this knowledge will inform the implementation of evidence-based rehabilitation interventions.