Over the last two decades, our understanding of the pathogenesis of central line associated blood steam infections has improved significantly. Also, increased attention has been focused on reducing healthcare worker exposure to infectious agents. Best practice protocols have been developed to eliminate unnecessary morbidity, mortality and costs associated with these infections and exposures. Adoption of these best practices has been incomplete and non-compliance is a major factor preventing our infectious complication rates from reaching zero. Getting at the root cause of noncompliance is complex. Online surveys are uniquely positioned to help understand the human factors contributing to non-compliance. This article reviews some of the pros and cons associated with the use of online surveys. Using several relevant recent examples, this article explores how these surveys can be used to identify those factors that create barriers to compliance. By better understanding all the issues involved with non-compliance, we will be able to create strategies and engineer products to improve best practice protocol compliance and reduce the human factor contribution to our patient's infectious complications.