Purpose: The objective was to evaluate the antagonistic effect of Lactobacillus and Bifidobacterium recovered from five commercial probiotics on the growth of C. albicans. Study design: The Lactobacillus and Bifidobacterium strains of five commercial probiotics were recovered and grown: Probio Hp+®, ProBiseis®, Lactipan®, Liolactil®, and Lacteol Fort®; 50 mg of each was hydrated and grown in Lactobacilli MRS (De Man, Rogosa and Sharpe) broth and incubated at 37°C with stirring (120 RPM) for 24 hours. Serial dilutions of 10−1 to 10−7 were made and viability was verified and quantified. For the antagonism tests, a suspension/inoculum of Lactobacillus strains recovered from each commercial preparation (4–30 × 109) and C. albicans ATCC 90028 (1.5–8 × 108) was prepared in MRS broth and incubated for 48 hours at 36°C, then plated on Dextrose Sabouraud Agar with Chloramphenicol and Rogosa Agar and the colony-forming units (CFU) were quantified. Additionally, viability was evaluated using the LIVE/DEAD® Yeast and Bacterial Viability kit. Results: The probiotic that produced the highest acidity of the medium was Lactipan®, followed by Probiseis® and Liolactil®, while Probio Hp+® showed the least change. Probiseis® was determined to have the highest growth of probiotic bacteria and the highest inhibition on C. albicans, followed by Lactipan®; Liolactil® and ProbioHp+® showed the least effect. In fluorescence tests, ProBiseis® showed the best effect, followed by Liolactil® and Lactipan®; Probio Hp+® had less of an effect. Conclusions: Two commercial products (ProBiseis and Lactipan) whose formulations have L. acidophilus, L. casei, L. rhamnosus, L. plantarum, B. infantis, and S. thermophilus have a greater inhibitory effect on C. albicans ATCC 90028
Skip Nav Destination
Close
Article navigation
Research Article|
November 12 2020
Effect of Five Commercial Probiotic Formulations on Candida Albicans Growth: In Vitro Study
Liz Mariana Hernández-Bautista;
Liz Mariana Hernández-Bautista
*
Search for other works by this author on:
Luis Octavio Sánchez-Vargas
Luis Octavio Sánchez-Vargas
******
Send all Correspondence to: Luis Octavio Sánchez Vargas, Biochemical and Microbiology Laboratory, Faculty of Stomatology, Autonomous University of San Luis Potosí, Av. Dr. Manuel Nava #2, Zona Universitaria, 78290, S.L.P. México. Phone: 524448262357, Fax: 524448139743. E-mail: octavio.sanchez@uaslp.mx,
Search for other works by this author on:
J Clin Pediatr Dent (2020) 44 (5): 315–322.
Citation
Liz Mariana Hernández-Bautista, Raúl Márquez-Preciado, Marine Ortiz-Magdaleno, Amaury Pozos-Guillén, Saray Aranda-Romo, Luis Octavio Sánchez-Vargas; Effect of Five Commercial Probiotic Formulations on Candida Albicans Growth: In Vitro Study. J Clin Pediatr Dent 1 January 2020; 44 (5): 315–322. doi: https://doi.org/10.17796/1053-4625-44.5.5
Download citation file:
Close
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Sign in via your Institution
Sign in via your Institution.gif?versionId=2249)
2
Views
0
Citations
Citing articles via
Erosive Tooth Wear, Presence of Parafunctional Habits and Tooth Injuries–Occurrence in a Group of Children and Adolescents Exposed to Domestic Violence
Anna Maria Pantelewicz, Dorota Olczak-Kowalczyk
Measurement of the Maximum Occlusal Bite Force and its Relation to the Caries Spectrum of First Permanent Molars in Early Permanent Dentition
Ravi Kumar Gudipaneni, Mohammad Khursheed Alam, Santosh R Patil, Mohmed Isaqali Karobari
Bilateral Condylar Fracture: A Case Treated with a Conservative Myofunctional Approach
Antonio Bedoya-Rodriguez, German O Ramirez-Yanez
Effects of Combined Rapid Maxillary Expansion and Facemask Therapy on the Mandibular Dental Arch in Mixed Dentition
Fatma Deniz Uzuner, Tuba Tortop, Şenol Gülşen, Selin Kale Varlık