Totarol, a diterpenoid phenol isolated from Podocarpus totara (D. Don ex Lambert) root bark, was found to be active against second- and fourth-instar Culex pipiens Coquillett with the 24 h LC50 values of 0.25 and 0.37 μg/mL, respectively. The mosquito larvicidal activity against C. pipiens increased when bioassays were extended to 48 h. Structural elucidation of totarol was by means of 1H-NMR, 13C-NMR, and GC-MS analysis.

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Author notes

3Department of Agricultural Chemistry and Soil Science, The University of Sydney, Sydney, NSW 2006, Australia.

4Department of Agricultural Chemistry, Korea University, Seoul 136-701, South Korea.