We investigated whether the co-occurrence of phytopathogens ( Clavibacter michiganensis subsp. michiganensis [ Cmm ] and Xanthomonas gardneri [ Xg ]) frequently encountered in tomato production and Salmonella enterica subsp. enterica serotype Typhimurium (JSG626) affects the persistence of these pathogens in tomato plant tissues during the early stages of plant growth . We observed that Cmm increased the persistence of Salmonella Typhimurium JSG626 (up to 1.8-log CFU/plant at 21 days post-inoculation; DPI) from co-inoculated tomato plants compared to plants inoculated with S. Typhimurium JSG626 alone (P<0.05). Xg had no effect on S. Typhimurium JSG626 persistence in planta . The increased persistence of S. Typhimurium JSG626 was also observed when it was inoculated seven days after Cmm inoculation of the same plant tissues (P<0.05). On the other hand, S. Typhimurium JSG626 reduced the population of both Cmm and Xg (up to 1.5-log CFU/plant at 21DPI; P<0.05) in co-inoculated plants group compared to plants inoculated with Cmm or Xg alone. Further, the Xg population increased (1.16-log CFU/plant at 21DPI; P<0.05) when S. Typhimurium JSG626 was inoculated seven days after Xg inoculation compared to plants inoculated with Xg alone. Our findings demonstrate that the type of phytopathogen present in the phyllosphere and inoculation time influence the persistence of S. Typhimurium JSG626 and its interactions with phytopathogens co-colonized in planta . Further, Salmonella seems to reduce the phytopathogen load in planta , while Cmm enhances the persistence of Salmonella from the co-inoculated plant tissues; however, further investigations are needed to understand mechanisms behind these interactions.
Impact of plant pathogens infection on Salmonella enterica subsp. enterica serotype Typhimurium persistence in tomato plants
- Views Icon Views
- Share Icon Share
- Search Site
Gireesh Rajashekara, Loic Deblais, Sally A Miller; Impact of plant pathogens infection on Salmonella enterica subsp. enterica serotype Typhimurium persistence in tomato plants. J Food Prot doi: https://doi.org/10.4315/JFP-20-291
Download citation file: