Thirty-three strains of bacteria were isolated during routine analysis of dairy products at two Canadian centers from colonies on violet red bile agar VRB which measured less than 0.5 mm in diameter after incubation at 35 C for 24 hr. All isolates showed typical coli-form morphology and Gram reaction and from the results of biochemical test were identified as coliform biotypes. No strain survived laboratory pasteurization at 145 F for 30 min. Strains of presumptive intestinal orgin (E. coli) were typed serologically and one strain was found to be of serotype 026:B6, an important etiological agent of infantile diarrhoea. It is concluded that size of colony is not a valid criterion for discriminating between colonies of coliform and non-coliform bacteria on VRB.
1This investigation was supported by funds provided by the Public Health Research Grant (No. 607-7-58) of the National Health Grants Program.