About a pint (550 ml) of yellow staining milk was filtered using vacuum levels of 76 and 381 mm of Hg through Lintine sediment disks which weighed 65, 90, or 112 mg. The time taken for the milk to flow through the disks was longer with heavier disks and the lower vacuum level. The fat retained on the disks and the concentration of β-carotene in that fat increased with disk weight.

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