Thirty food samples were frozen and stored at −18, −26 and −34 C. Subsamples were analyzed for changes in total aerobic flora, coliforms and fungi after 0.5, 1, 2, 4 and 6 months. Fungal populations were determined using both acidified Potato Dextrose Agar (PDA) and antibiotic Plate Count Agar (SPCA). Analysis of the data showed that coliforms, as measured by Violet Red Bile Agar, exhibited the greatest decrease during storage, whereas, the fungal population exhibited a sensitivity to freezing greater or less than the total aerobic flora, depending upon the medium used. Since PDA does not accurately measure the total fungal population, the SPCA count may reflect the true fate of fungi during frozen storage.
1Florida Agricultural Experiment Stations Journal Series No. 2555.