Mucor racemosus isolated from butter exhibited maximum lipase production at 22 C in 3 days at pH 5.0, when grown as a static culture. Supplementation of the medium with glucose and lactose at 1% level resulted in maximum (18.0 μmoles FFA) and minimum (10.5 μmoles FFA) enzyme production. Of the five different nitrogen sources tested, peptone at the 2% level supported highest production of lipase. Calcium, potassium and sodium citrate (0.1 %), when incorporated in the medium, stimulated production of enzyme to the extent of 80, 60 and 47%, respectively.

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