Listeria monocytogenes ATCC 19115, Scott A, and UAL500 were evaluated for sensitivity to nisin (0 to 50 μg/ml) using a direct plating method. Nisin (10 μg/ml) decreased an initial population of L. monocytogenes (109 CFU per ml) by 6- to 7-log cycles. Sensitivity to nisin was enhanced by addition of 2% NaCl or by reduction of the medium pH from 6.5 to 5.5 with either hydrochloric or lactic acid. Mutants resistant to 50 μg/ml nisin were detected at frequencies of 10−6 to 10−8. Nisin-resistant L. monocytogenes mutants should be expected to arise when nisin is used as an antimicrobial in food systems.
5Present address: Department of Food Science, University of Guelph, Guelph, Ontario, Canada N1G 2W1