A microtiter plate format previously reported for the biochemical characterization of microorganisms was modified by adding agar to all of the different test media instead of using a combination of liquid and solid media. This modification, termed the Modified Microtiter Plate procedure (MMP), offered the same advantages of the original method (labor saving, inexpensive, and custom designed for special needs) as well as having the added advantages of longer term storage prior to use and ease of handling. In this study, 60 bacterial isolates (both Listeria suspects and known Listeria cultures) were biotyped using the MMP protocol and compared to results obtained using the classical tube-based US-FDA recommended protocol as the standard identification method and the BBL-Minitek system.

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