A variety of unacidified products bottled in oil or water were investigated for their ability to support growth and toxin production by Clostridium botulinum. The products were inoculated with a mixture of five strains of C. botulinum type A spores (about 50 spores/g or ml) and incubated at room temperature (23°C). At monthly intervals the organoleptic acceptability of the products, as determined by appearance, odor, and texture, was evaluated and a portion of each sample was removed, diluted 1:2 in gel-phosphate buffer, and injected intraperitoneally into mice. At the end of 4 months the drained solids of each sample were macerated with a minimal amount of buffer and centrifuged; the clear extracts were then injected into mice. None of the products tested supported growth and toxin production by C. botulinum.
Evaluation of Unacidified Products Bottled in Oil for Outgrowth and Toxin Production by Clostridium botulinum
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HAIM M. SOLOMON, DONALD A. KAUTTER, E. JEFFERY RHODEHAMEL, TIMOTHY LILLY; Evaluation of Unacidified Products Bottled in Oil for Outgrowth and Toxin Production by Clostridium botulinum. J Food Prot 1 August 1991; 54 (8): 648–649. doi: https://doi.org/10.4315/0362-028X-54.8.648
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