The predominant fungal species present in 60 samples of barley collected in Spain were Alternaria alternata, Penicillium spp. and Aspergillus flavus. Of the 176 Alternaria isolates examined, 88.6% produced tenuazonic acid, 15.3% produced alternariol, and 9% produced alternariol monomethyl ether. Only 6% of the 190 isolates of A. flavus produced aflatoxin.

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