Four commercial emulsifiers, sodium oleate, sodium stearate, sodium stearoyl-2-lactylate, and calcium stearoyl-2-lactylate, were evaluated for their effectiveness as inhibitors of Pseudomonas lipases. Each emulsifier was incubated at 0.5, 1.0, and 2.0% concentrations with the individual as well as a mixture of the crude lipase extracts obtained from three Pseudomonas spp. (P. fluorescens, P. putida, and P. cepacia), in reconstituted nonfat dry milk (10%) for 30 min. At a 2% concentration, all emulsifiers tested were potent inhibitors (100%) of lipase activity. The effectiveness of lipolysis inhibition declined with decreasing emulsifier concentration. Sodium oleate demonstrated significant (P < 0.05) lipolysis inhibition at a 1% concentration regardless of initial lipase level. Synergism in inhibiting lipolytic activity between these selected emulsifiers was not evident.

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Author notes

Published as Paper No. 8922, Journal Series Nebraska Agricultural Experiment Station, Lincoln, NE 68583-0919.