Campylobacter jejuni is a thermophilic and microaerophilic enteric pathogen associated with poultry. Biofilms may be a source of C. jejuni in poultry house water systems since they can protect constituent microorganisms from environmental stress. In this study, the viability of C. jejuni in biofilms of gram-positive chicken house isolates (P1, Y1, and W1) and a Pseudomonas sp. was determined using a cultural method (modified brucella agar) and direct viable count (DVC). Two-day biofilms grown on polyvinyl chloride (PVC) coupons in R2A broth at 12 and 23°C were incubated with C. jejuni for a 6-h attachment period. Media were then refreshed every 24 h for 7 days to allow biofilm growth. Two-day biofilms of P1, Y1, and Pseudomonas spp. enhanced attachment (P < 0.01) of C. jejuni (4.74, 4.62, and 4.78 log cells/cm2, respectively)compared to W1 and controls without preexisting biofilm (4.31 and 4.22 log cells/cm2, respectively). On day 7, isolates P1 and Y1 and Pseudomonas biofilms covered 5.4, 7.0, and 21.5% of the surface, respectively, compared to 4.9% by W1. Viable C. jejuni on the surface decreased (P < 0.05) with time, with the greatest reduction occurring on surfaces without a preexisting biofilm. The number of viable C. jejuni determined by DVC was greater than that determined by the cultural method, indicating that C. jejuni may form a viable but nonculturable state within the biofilm. Both DVC and the cultural method indicate that biofilms enhance (P < 0.01) the survival of C. jejuni during incubation at 12 and 23°C over a 7-day period.
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Research Article| July 01 2002
Survival of Campylobacter jejuni in Biofilms Isolated from Chicken Houses
J. F. FRANK;
J. F. FRANK *
1Center for Food Safety, Department of Food Science and Technology, University of Georgia, Athens, Georgia 30605-2106
*Author for correspondence. Tel: 706-542-0994; Fax: 706-542-1050; E-mail: firstname.lastname@example.org.
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N. TRACHOO, J. F. FRANK, N. J. STERN; Survival of Campylobacter jejuni in Biofilms Isolated from Chicken Houses. J Food Prot 1 July 2002; 65 (7): 1110–1116. doi: https://doi.org/10.4315/0362-028X-65.7.1110
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