Pulsed UV light is a novel technology to inactivate pathogenic and spoilage microorganisms in a short time. The efficacy of pulsed UV light (5.6 J/cm2 per pulse) for the inactivation of Staphylococcus aureus as suspended or agar seeded cells was investigated. A 12-, 24-, or 48-ml cell suspension in buffer was treated under pulsed UV light for up to 30 s, and 0.1 ml of sample was surface plated on Baird-Parker agar and incubated at 37° C for 24 h to determine log reductions. Also, 0.1 ml of cell suspension in peptone water was surface plated on Baird-Parker agar plates, and the plates were treated under pulsed UV light for up to 30 s. The treated and untreated plates were incubated in the conditions described above. A 7- to 8-log CFU/ml reduction was observed for suspended and agar-seeded cells treated for 5 s or longer. In the case of suspended cells, the sample depth, time, treatment, and interaction were significant (P < 0.05). In the case of agar-seeded cells, the treatment time was significant (P < 0.05). Our results clearly indicate that pulsed UV technology has potential for the inactivation of pathogenic microorganisms.
Research Article| May 01 2004
Inactivation of Staphylococcus aureus by Pulsed UV-Light Sterilization
ALI DEMIRCI *
1Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
2The Huck Institutes of Life Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802, USA
*Author for correspondence. Tel: 814-863-1098; Fax: 814-863-1031; E-mail: firstname.lastname@example.org.
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KATHIRAVAN KRISHNAMURTHY, ALI DEMIRCI, JOSEPH IRUDAYARAJ; Inactivation of Staphylococcus aureus by Pulsed UV-Light Sterilization. J Food Prot 1 May 2004; 67 (5): 1027–1030. doi: https://doi.org/10.4315/0362-028X-67.5.1027
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