Zearalenone is an estrogenic mycotoxin commonly found in grains throughout the world. A number of instrument- and antibody-based methods including enzyme-linked immunosorbent assays (ELISAs) have been developed to detect zearalenone (ZEN) and related toxins in commodities and foods. Although convenient, the commercial ELISAs for small molecules such as ZEN require a washing step to separate bound and unbound enzyme label before detection. In fluorescence polarization immunoassays, separation of bound and unbound label is not required, a property that reduces the time needed to perform the assays. We developed a fluorescence polarization immunoassay for ZEN in maize. When combined with a rapid extraction technique, the assay could be used to detect as little as 0.11 μg of ZEN g−1 maize within 10 min. The assay showed cross-reactivity to the ZEN analogs zearalanone, α-zearalanol, α-zearalenol, β-zearalenol, and β-zearalanol of 195, 139, 102, 71, and 20%, respectively, relative to ZEN (100%). Recovery of ZEN from spiked maize over the range of 0.5 to 5 μg g−1 averaged 100.2% (n = 12). The fluorescence polarization immunoassay results were comparable to those obtained with a liquid chromatographic method for the analysis of 60 naturally contaminated maize samples and maize samples amended with culture material. The fluorescence polarization immunoassay provides a rapid method for screening of maize for ZEN.
Research Article| May 01 2004
Detection of Zearalenone and Related Metabolites by Fluorescence Polarization Immunoassay
CHRIS M. MARAGOS;
CHRIS M. MARAGOS *
Mycotoxin Research Unit, National Center for Agricultural Utilization Research, U.S. Department of Agriculture, Agricultural Research Service, 1815 North University Street, Peoria, Illinois 61604, USA
*Author for correspondence. Fax: 309-681-6689; E-mail: email@example.com.
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CHRIS M. MARAGOS, EUN-KYUNG KIM; Detection of Zearalenone and Related Metabolites by Fluorescence Polarization Immunoassay. J Food Prot 1 May 2004; 67 (5): 1039–1043. doi: https://doi.org/10.4315/0362-028X-67.5.1039
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