Survival of naturally occurring larvae of Anisakis simplex in fresh arrowtooth flounder (Atheresthes stomia) was determined after storage for specified periods at four freezing temperatures. All larvae were killed by 96, 60, 12, and 9 h at temperatures of −15, −20, −30, and −40°C, respectively. The average percentages of live larvae per fillet at the next shortest holding time were as follows: 72 h at −15°C, 0 to 3%; 48 h at −20°C, 11 to 30%; 9 h at −30°C, 5%; and 6 h at −40°C, 0 to 3%. Larval survival was directly related to fillet thickness or weight (P ≤ 0.05). Larval death was directly correlated to freezing temperatures. Holding time necessary to kill larval nematodes decreased as storage temperature decreased.

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Author notes

The information and conclusions in this manuscript do not represent new policy and regulations of the U.S. Food and Drug Administration, nor do they imply an imminent change in existing policy and regulations.

Present address: U.S. Food and Drug Administration, Center for Veterinary Medicine, 8401 Muirkirk Road, Laurel, MD 20708, USA.

§ Present address: Department of Food Science and Human Nutrition, University of Illinois, 260 Bevier Hall, 905 South Goodwin Avenue, Urbana, IL 61801, USA.