The spread and persistence of Listeria monocytogenes in smoked fish products and seafood processing factories are big concerns. Thus, the corresponding quality assurance programs must include adequate microbiological control measures. We evaluated eight different pre-PCR sample processing strategies to be coupled with a previously developed real-time PCR assay for the quantitative detection of L. monocytogenes in salmon products. The optimal pre-PCR procedure involved filtration and DNA purification with the use of a commercial kit. This strategy could detect 10 CFU of L. monocytogenes per g of smoked salmon and could quantify 1,000 CFU/g with excellent accuracy compared with the standard plate count method. Thus, this method could be a promising alternative for the quantitative detection of L. monocytogenes in smoked fish products and processing factories. This method could also detect the bacterium in raw salmon.
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Research Article|
July 01 2005
Rapid Quantitative Detection of Listeria monocytogenes in Salmon Products: Evaluation of Pre–Real-Time PCR Strategies
DAVID RODRÍGUEZ-LÁZARO;
DAVID RODRÍGUEZ-LÁZARO
1Institute of Food and Agricultural Technology (INTEA), University of Girona, Campus Montilivi (edif. Politècnica1), E-17071 Girona, Spain
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ANNA JOFRÉ;
ANNA JOFRÉ
2Institute for Food and Agricultural Research and Technology (IRTA), Meat Technology Center, Granja Camps i Armet E-17121 Monells, Spain
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TERESA AYMERICH;
TERESA AYMERICH
2Institute for Food and Agricultural Research and Technology (IRTA), Meat Technology Center, Granja Camps i Armet E-17121 Monells, Spain
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MARGARITA GARRIGA;
MARGARITA GARRIGA
2Institute for Food and Agricultural Research and Technology (IRTA), Meat Technology Center, Granja Camps i Armet E-17121 Monells, Spain
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MARIA PLA
MARIA PLA
*
1Institute of Food and Agricultural Technology (INTEA), University of Girona, Campus Montilivi (edif. Politècnica1), E-17071 Girona, Spain
* Author for correspondence. Tel: +34-972 419852; Fax: +34-972 418399; E-mail: maria.pla@udg.es.
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J Food Prot (2005) 68 (7): 1467–1471.
Article history
Received:
September 29 2004
Accepted:
February 05 2005
Citation
DAVID RODRÍGUEZ-LÁZARO, ANNA JOFRÉ, TERESA AYMERICH, MARGARITA GARRIGA, MARIA PLA; Rapid Quantitative Detection of Listeria monocytogenes in Salmon Products: Evaluation of Pre–Real-Time PCR Strategies. J Food Prot 1 July 2005; 68 (7): 1467–1471. doi: https://doi.org/10.4315/0362-028X-68.7.1467
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