The outgrowth of spoilage organisms, including molds and yeasts, results in significant financial loss to the food industry and wastes natural resources. The objective of this study was to develop a rapid, specific, and sensitive real-time PCR method for detecting spoilage molds during screening of raw materials and final product quality control analysis. The 18S rRNA gene was used to develop PCR primers and probe. With this set of primers and probe, less than 1,000 mold cells per milliliter of orange juice (10 cells per reaction) were detected with the real-time PCR system within 6 to 7 h. No cross-reactivity was found with other common foodborne bacteria, yeasts, or food ingredients. This technique is significantly faster than current detection and identification procedures, which take from days to weeks.
Skip Nav Destination
Article navigation
Research Article|
February 01 2006
Rapid, Specific, and Sensitive Detection of Spoilage Molds in Orange Juice Using a Real-Time Taqman PCR Assay
KAI WAN;
KAI WAN
1Department of Food Science and Technology, Ohio State University, Columbus, Ohio 43210, USA
Search for other works by this author on:
AHMED E. YOUSEF;
AHMED E. YOUSEF
1Department of Food Science and Technology, Ohio State University, Columbus, Ohio 43210, USA
2Department of Microbiology, Ohio State University, Columbus, Ohio 43210, USA
Search for other works by this author on:
STEVE J. SCHWARTZ;
STEVE J. SCHWARTZ
1Department of Food Science and Technology, Ohio State University, Columbus, Ohio 43210, USA
Search for other works by this author on:
HUA H. WANG
HUA H. WANG
*
1Department of Food Science and Technology, Ohio State University, Columbus, Ohio 43210, USA
2Department of Microbiology, Ohio State University, Columbus, Ohio 43210, USA
* Author for correspondence. Tel: 614-292-0579; Fax: 614-292-0218; E-mail: wang.707@osu.edu.
Search for other works by this author on:
J Food Prot (2006) 69 (2): 385–390.
Article history
Received:
April 18 2005
Accepted:
July 21 2005
Citation
KAI WAN, AHMED E. YOUSEF, STEVE J. SCHWARTZ, HUA H. WANG; Rapid, Specific, and Sensitive Detection of Spoilage Molds in Orange Juice Using a Real-Time Taqman PCR Assay. J Food Prot 1 February 2006; 69 (2): 385–390. doi: https://doi.org/10.4315/0362-028X-69.2.385
Download citation file: