A phage replication–competitive enzyme-linked immunosorbent assay (PR-cELISA) was developed for the detection of multiple antibiotic–resistant Salmonella Typhimurium DT104. In the PR-cELISA procedure, a phage, BP1, was inoculated into a log-phase bacterial culture at a ratio of 1:100. After a 3-h incubation of the mixture, BP1 replication was measured by cELISA based on the competitive binding between BP1 and biotinylated BP1 to Salmonella Typhimurium smooth lipopolysaccharide. Among the 84 Salmonella strains and 9 non-Salmonella strains that were tested by PR-cELISA, BP1 detected 39 of 40 Salmonella Typhimurium strains, 2 of 10 Salmonella non-Typhimurium somatic group B strains, and 5 of 18 Salmonella somatic group D1 strains. With the addition of chloramphenicol to the culture medium, PR-cELISA detected all 27 multiple antibiotic–resistant Salmonella Typhimurium DT104 and none of the other Salmonella strains or non-Salmonella strains tested. The results demonstrated that PR-cELISA has potential applications for the detection of multiple antibiotic–resistant Salmonella Typhimurium DT104.
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Research Article|
April 01 2006
Detection of Multiple Antibiotic–Resistant Salmonella enterica Serovar Typhimurium DT104 by Phage Replication–Competitive Enzyme-Linked Immunosorbent Assay
JIEWEN GUAN;
JIEWEN GUAN
*
1Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, Ontario, Canada K2H 8P9
* Author for correspondence. Tel: 613-2286698; Fax: 613-2286670; E-mail: guanj@inspection.gc.ca.
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MARIA CHAN;
MARIA CHAN
1Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, Ontario, Canada K2H 8P9
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BÉATRICE ALLAIN;
BÉATRICE ALLAIN
2Biophage Pharma Inc., 6100 Royalmount Avenue, Montreal, Québec, Canada H4P 2R2
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ROSEMONDE MANDEVILLE;
ROSEMONDE MANDEVILLE
2Biophage Pharma Inc., 6100 Royalmount Avenue, Montreal, Québec, Canada H4P 2R2
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BRIAN W. BROOKS
BRIAN W. BROOKS
1Ottawa Laboratory Fallowfield, Canadian Food Inspection Agency, 3851 Fallowfield Road, Nepean, Ontario, Canada K2H 8P9
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J Food Prot (2006) 69 (4): 739–742.
Article history
Received:
August 31 2005
Accepted:
November 11 2005
Citation
JIEWEN GUAN, MARIA CHAN, BÉATRICE ALLAIN, ROSEMONDE MANDEVILLE, BRIAN W. BROOKS; Detection of Multiple Antibiotic–Resistant Salmonella enterica Serovar Typhimurium DT104 by Phage Replication–Competitive Enzyme-Linked Immunosorbent Assay. J Food Prot 1 April 2006; 69 (4): 739–742. doi: https://doi.org/10.4315/0362-028X-69.4.739
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