An enzyme-linked immunosorbent assay (ELISA), immunochromatography (ICG) strip test, and immunomagnetic bead separation (IMBS) system based on a monoclonal antibody were individually developed for the detection and isolation of Listeria monocytogenes in meat samples. The three methods showed a strong reaction with Listeria species and a weak reaction with Staphylococcus aureus. To increase the rapidity of L. monocytogenes detection, combinations of the ELISA and ICG strip test with the IMBS system (ELISA-IMBS and ICG-IMBS) were investigated. In comparative analyses of artificially inoculated meat and samples of processed meat, the ELISA and ICG strip test required 24 h of enrichment time to detect the inoculated meat samples with ≥ 1 × 102 CFU/10 g, whereas the ELISA-IMBS and ICG-IMBS required only 14 h of enrichment. Analyses of naturally contaminated meat samples (30 pork samples, 20 beef samples, 26 chicken samples, 20 fish samples, and 20 processed meat samples) performed by ELISA-IMBS, ICG-IMBS, and API kit produced similar results. The ELISA-IMBS and ICG-IMBS provide a more rapid assay than the individual ELISA and the ICG strip test and are appropriate for rapid and qualitative detection of L. monocytogenes (or Listeria species) in meat samples. With the ICG-IMBS, L. monocytogenes could be detected in meat samples within 15 h and the method has potential as a rapid, cost-effective on-site screening tool for the detection of L. monocytogenes in food samples and agricultural products at a minimum detection level of ~100 CFU/10 g.
Skip Nav Destination
Article navigation
Research Article|
April 01 2008
Enhanced Rapidity for Qualitative Detection of Listeria monocytogenes Using an Enzyme-Linked Immunosorbent Assay and Immunochromatography Strip Test Combined with Immunomagnetic Bead Separation
WON-BO SHIM;
WON-BO SHIM
1Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University, Chinju, Gyeongnam 660-701, Korea
2Division of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University, 119992 Moscow, Russia
Search for other works by this author on:
JIN-GIL CHOI;
JIN-GIL CHOI
1Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University, Chinju, Gyeongnam 660-701, Korea
Search for other works by this author on:
JI-YOUNG KIM;
JI-YOUNG KIM
1Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University, Chinju, Gyeongnam 660-701, Korea
Search for other works by this author on:
ZHENG-YOU YANG;
ZHENG-YOU YANG
1Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University, Chinju, Gyeongnam 660-701, Korea
Search for other works by this author on:
KYU-HO LEE;
KYU-HO LEE
3Department of Environmental Engineering and Biotechnology, Hankuk University of Foreign Studies, Yongin, Gyunggi 449-791, Korea
Search for other works by this author on:
MIN-GON KIM;
MIN-GON KIM
4Laboratory of Integrative Biotechnology, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-333, Korea
Search for other works by this author on:
SANG-DO HA;
SANG-DO HA
5Department of Food Science and Technology, Chung-Ang University, 72-1 Naeri, Ansung, Gyunggi 456-756, Korea
Search for other works by this author on:
KEUN-SUNG KIM;
KEUN-SUNG KIM
5Department of Food Science and Technology, Chung-Ang University, 72-1 Naeri, Ansung, Gyunggi 456-756, Korea
Search for other works by this author on:
KWANG-YUP KIM;
KWANG-YUP KIM
6Department of Food Science and Technology, Chungbuk University, Cheongju, Chungbuk 361-764, Korea
Search for other works by this author on:
CHEOL-HO KIM;
CHEOL-HO KIM
7Department of Biological Sciences, Sungkyunkwan University, Chunchun-Dong 300, Suwon, Gyunggi 440-746, Korea
Search for other works by this author on:
SERGEI A. EREMIN;
SERGEI A. EREMIN
2Division of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University, 119992 Moscow, Russia
Search for other works by this author on:
DUCK-HWA CHUNG
DUCK-HWA CHUNG
*
1Division of Applied Life Science (Brain Korea 21 program), Graduate School of Gyeongsang National University, Chinju, Gyeongnam 660-701, Korea
* Author for correspondence. Tel: +82-55-751-5480; Fax: +82-55-757-5485; E-mail: dhchung@gnu.ac.kr.
Search for other works by this author on:
J Food Prot (2008) 71 (4): 781–789.
Article history
Received:
April 27 2007
Accepted:
December 15 2007
Citation
WON-BO SHIM, JIN-GIL CHOI, JI-YOUNG KIM, ZHENG-YOU YANG, KYU-HO LEE, MIN-GON KIM, SANG-DO HA, KEUN-SUNG KIM, KWANG-YUP KIM, CHEOL-HO KIM, SERGEI A. EREMIN, DUCK-HWA CHUNG; Enhanced Rapidity for Qualitative Detection of Listeria monocytogenes Using an Enzyme-Linked Immunosorbent Assay and Immunochromatography Strip Test Combined with Immunomagnetic Bead Separation. J Food Prot 1 April 2008; 71 (4): 781–789. doi: https://doi.org/10.4315/0362-028X-71.4.781
Download citation file: