The aim of this work was to examine various purchased meat products and to find out if any traces of Mycobacterium avium subsp. avium, M. avium subsp. hominissuis, and M. avium subsp. paratuberculosis could be detected in these samples. Analysis of the meat products (raw, cooked, and fermented) was performed using a real-time quantitative PCR (qPCR) method for the detection of specific insertion sequences: duplex qPCR for the detection of IS900 specific for M. avium subsp. paratuberculosis, and triplex qPCR for the detection of IS901 specific for Mycobacterium avium subsp. avium and IS1245 specific for M. avium subsp. hominissuis. Of the 77 analyzed meat samples, 17 (22%) were found to contain M. avium subsp. paratuberculosis DNA, 4 (5%) samples contained Mycobacterium avium subsp. avium DNA, and in 12 (16%) samples M. avium subsp. hominissuis DNA was detected. The concentration of M. avium subsp. paratuberculosis and M. avium subsp. hominissuis DNA in some meat products exceeded 104 genomes per g. Culture examination of these mycobacterial subspecies was negative. By analyzing a range of meat products, we have provided evidence to support the hypothesis that M. avium is present in everyday commodities sold to the general public
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Research Article|
April 01 2011
Real-Time Quantitative PCR Detection of Mycobacterium avium Subspecies in Meat Products
B. KLANICOVA;
B. KLANICOVA
Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
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I. SLANA;
I. SLANA
Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
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H. VONDRUSKOVA;
H. VONDRUSKOVA
Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
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M. KAEVSKA;
M. KAEVSKA
Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
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I. PAVLIK
I. PAVLIK
*
Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic
* Author for correspondence. Tel: +420 5 3333 1601; Fax: +420 5 4121 1229; E-mail: pavlik@vri.cz.
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J Food Prot (2011) 74 (4): 636–640.
Article history
Received:
August 10 2010
Accepted:
December 03 2010
Citation
B. KLANICOVA, I. SLANA, H. VONDRUSKOVA, M. KAEVSKA, I. PAVLIK; Real-Time Quantitative PCR Detection of Mycobacterium avium Subspecies in Meat Products. J Food Prot 1 April 2011; 74 (4): 636–640. doi: https://doi.org/10.4315/0362-028X.JFP-10-332
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