Given the increasing number of antibiotic-resistant bacteria and the need to synthesize new antimicrobials, silver has attracted interest in the scientific community because of its recognized antimicrobial activity. This study aimed to evaluate the antimicrobial effects of silver nanoparticles (NP) obtained by a new method and tested at concentrations of 6 μg/ml and 60 μg/ml against the species Staphylococcus aureus, Listeria innocua, Salmonella Choleraesuis, Pseudomonas aeruginosa, Escherichia coli, and Bacillus cereus. The ability of these nanoparticles to remove or kill vegetative cells adhered to stainless steel surfaces was also evaluated. We observed that the NP obtained with the new method, concentrated silver nanoparticles (CNP), and silver nanoparticles with added sodium chloride (NPNaCl) had high antimicrobial activities (P < 0.05). We also verified that the most effective condition for the removal of P. aeruginosa cells on stainless steel coupons (10 by 10 mm) was immersion of the surfaces in CNP. The CNP treatment produced a 5-log reduction of the microbial population after 30 to 60 min of immersion. The CNP treatment also performed better than water and sodium carbonate, a compound commonly applied in clean-in-place procedures in the food industry, in removing adherent B. cereus cells from stainless steel cylinders. Therefore, these results suggest that NP synthesized by a new procedure may be used as antimicrobials in the food industry, for example, for the sanitization of utensils that come into contact with foods.
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Research Article|
April 01 2012
Antimicrobial Effects of Silver Nanoparticles against Bacterial Cells Adhered to Stainless Steel Surfaces
EMILIANE A. ARAÚJO;
EMILIANE A. ARAÚJO
1Food Technology Department, Federal University of Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil
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NÉLIO J. ANDRADE;
NÉLIO J. ANDRADE
*
1Food Technology Department, Federal University of Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil
* Author for correspondence. Tel: 55 031 31 3899 1682; Fax: 55 031 31 3899 2208; E-mail: nandrade@ufv.br.
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LUIS HENRIQUE M. da SILVA;
LUIS HENRIQUE M. da SILVA
2Chemistry Department, Federal University of Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil
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PATRÍCIA C. BERNARDES;
PATRÍCIA C. BERNARDES
1Food Technology Department, Federal University of Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil
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ÁLVARO V. N. de C. TEIXEIRA;
ÁLVARO V. N. de C. TEIXEIRA
3Physics Department, Federal University of Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil
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JOÃO PAULO N. de SÁ;
JOÃO PAULO N. de SÁ
1Food Technology Department, Federal University of Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil
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JOSÉ FELÍCIO Q. FIALHO, JR.;
JOSÉ FELÍCIO Q. FIALHO, JR.
1Food Technology Department, Federal University of Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil
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PATRÍCIA E. FERNANDES
PATRÍCIA E. FERNANDES
1Food Technology Department, Federal University of Viçosa, 36570-000, Viçosa, Minas Gerais, Brazil
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J Food Prot (2012) 75 (4): 701–705.
Article history
Received:
June 05 2011
Accepted:
November 20 2011
Citation
EMILIANE A. ARAÚJO, NÉLIO J. ANDRADE, LUIS HENRIQUE M. da SILVA, PATRÍCIA C. BERNARDES, ÁLVARO V. N. de C. TEIXEIRA, JOÃO PAULO N. de SÁ, JOSÉ FELÍCIO Q. FIALHO, PATRÍCIA E. FERNANDES; Antimicrobial Effects of Silver Nanoparticles against Bacterial Cells Adhered to Stainless Steel Surfaces. J Food Prot 1 April 2012; 75 (4): 701–705. doi: https://doi.org/10.4315/0362-028X.JFP-11-276
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