Pathogenic contamination is a food safety concern. This study was conducted to investigate the efficacy of neutral electrolyzed water (NEW) in killing pathogens, namely, Vibrio parahaemolyticus, Vibrio vulnificus, Salmonella Enteritidis, and Escherichia coli in shrimp. Pure cultures of each pathogen were submerged separately in NEW containing five different chlorine concentrations: 10, 30, 50, 70, and 100 ppm. For each concentration, three submersion times were tested: 1, 3, and 5 min. The population of V. parahaemolyticus was rapidly reduced even at low concentrations, but prolonged contact times caused only a slight reduction. V. vulnificus was gradually inhibited with increasing NEW concentrations and contact times. For the V. parahaemolyticus applications of 70 ppm for 5 min and of 100 ppm for 3 min, each eliminated 7 log CFU/ml. For V. vulnificus, applications of 50 ppm for 3 min and 100 ppm for 1 min, each eliminated 7 log CFU/ml. Salmonella Enteritidis and E. coli were slightly reduced by NEW. Applications of 50 ppm for 15 min and 10 ppm for 30 min completely eliminated 4.16 log CFU/g of V. parahaemolyticus in inoculated shrimp, while only a 1-log CFU/g reduction of V. vulnificus was detected. Soaking shrimp in 10 ppm NEW for 30 min did not affect its sensory quality. Our results suggest NEW could be an alternative sanitizer to improve the microbiological quality of seafood.
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Research Article|
December 01 2014
Efficacy of Neutral Electrolyzed Water for Reducing Pathogenic Bacteria Contaminating Shrimp
PATTAMA RATANA-ARPORN;
PATTAMA RATANA-ARPORN
*
Department of Fishery Products, Faculty of Fisheries, Kasetsart University, 50 Ngamwongwan Road, Ladyao, Chatuchak Bangkok, 10900 Thailand; and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, 10900 Thailand
* Author for correspondence. Tel: 6629428644-5; Fax: 6629428644-5, Ext 12; E-mail: ffispmr@ku.ac.th.
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NARUEMON JOMMARK
NARUEMON JOMMARK
Department of Fishery Products, Faculty of Fisheries, Kasetsart University, 50 Ngamwongwan Road, Ladyao, Chatuchak Bangkok, 10900 Thailand; and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University, Bangkok, 10900 Thailand
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J Food Prot (2014) 77 (12): 2176–2180.
Article history
Received:
April 07 2014
Accepted:
July 14 2014
Citation
PATTAMA RATANA-ARPORN, NARUEMON JOMMARK; Efficacy of Neutral Electrolyzed Water for Reducing Pathogenic Bacteria Contaminating Shrimp. J Food Prot 1 December 2014; 77 (12): 2176–2180. doi: https://doi.org/10.4315/0362-028X.JFP-14-161
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