Biofilm-forming bacteria resident to food processing facilities are a food safety concern due to the potential of biofilms to harbor foodborne bacterial pathogens. When cultured together, Ralstonia insidiosa, a strong biofilm former frequently isolated from produce processing environments, has been shown to promote the incorporation of Escherichia coli O157:H7 into dual-species biofilms. In this study, interactions between E. coli O157:H7 and R. insidiosa were examined under different incubating conditions. Under static culture conditions, the incorporation of E. coli O157:H7 into biofilms with R. insidiosa was not significantly affected by either low incubating temperature (10°C) or by limited nutrient availability. Greater enhancement of E. coli O157:H7 incorporation in dual-species biofilms was observed by using a continuous culture system with limited nutrient availability. Under the continuous culture conditions used in this study, E coli O157:H7 cells showed a strong tendency of colocalizing with R. insidiosa on a glass surface at the early stage of biofilm formation. As the biofilms matured, E coli O157:H7 cells were mostly found at the bottom layer of the dual-species biofilms, suggesting an effective protection by R. insidiosa in the mature biofilms.
Effects of Environmental Parameters on the Dual-Species Biofilms Formed by Escherichia coli O157:H7 and Ralstonia insidiosa, a Strong Biofilm Producer Isolated from a Fresh-Cut Produce Processing Plant
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NANCY T. LIU, XIANGWU NOU, GARY R. BAUCHAN, CHARLES MURPHY, ALAN M. LEFCOURT, DANIEL R. SHELTON, Y. MARTIN LO; Effects of Environmental Parameters on the Dual-Species Biofilms Formed by Escherichia coli O157:H7 and Ralstonia insidiosa, a Strong Biofilm Producer Isolated from a Fresh-Cut Produce Processing Plant. J Food Prot 1 January 2015; 78 (1): 121–127. doi: https://doi.org/10.4315/0362-028X.JFP-14-302
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