This study evaluated the antibacterial effect of the combination of ɛ-polylysine (ɛ-PL) and nisin against Enterococcus faecalis strains. The combination of ɛ-PL and nisin showed synergistic antibacterial activity against three Enterococcus strains. Scanning electron microscopy and a membrane permeability assay revealed that the combined treatment with ɛ-PL and nisin synergistically damaged the cell morphology of E. faecalis strain R612Z1 cells. Both ɛ-PL and nisin can dissipate the transmembrane electric potential of E. faecalis R612Z1 cells, but these peptides did not affect the transmembrane pH gradient. The combination of ɛ-PL and nisin can produce a high reactive oxygen species level in E. faecalis R612Z1 cells. The results indicated that the uptake of ɛ-PL into cells was promoted through nisin and that the combination of ɛ-PL and nisin could produce a high reactive oxygen species level in E. faecalis R612Z1 cells, leading to cell growth inhibition.
Synergistic Antibacterial Effect of the Combination of ɛ-Polylysine and Nisin against Enterococcus faecalis
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FANG LIU, MEI LIU, LIHUI DU, DAOYING WANG, ZHIMING GENG, MUHAN ZHANG, CHONG SUN, XIAOXI XU, YONGZHI ZHU, WEIMIN XU; Synergistic Antibacterial Effect of the Combination of ɛ-Polylysine and Nisin against Enterococcus faecalis. J Food Prot 1 December 2015; 78 (12): 2200–2206. doi: https://doi.org/10.4315/0362-028X.JFP-15-220
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