The basic 12-disk Minitek system (14 tests) and the API 20E system were evaluated for identification of Yersinia enterocolitica strains isolated at various locations in the world. The API 20E system correctly identified all the Y. enterocolitica strains while the Minitek system identified 80% (20/25) of the strains. Four of the five cultures misidentified by Minitek were atypical strains isolated from vacuum-packaged meats. These four cultures were identified as Citrobacter freundii by Minitek due to a rhamnose-positive reaction. Minitek and API 20E are rapid and convenient systems which could prove to be beneficial for identifying Y. enterocolitica strains.