In order to study the effects of environmental contaminants on the sexual development of the cricket frog, Acris crepitans, a system of laboratory breeding of this species was devised using induction of mating by subcutaneous injection of synthetic human luteinizing hormone releasing hormone at doses ranging from 169–676 mg/kg body weight. Viable tadpoles were obtained from frogs that had been maintained in captivity for approximately 1.5 years and from recently captured individuals. The techniques and doses described represent a starting point for further studies aimed at optimizing captive breeding technique for this species

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