Characterization of PM10 and Impact on Human Health During the Annual Festival of Lights (Diwali)

Background. Diwali is a Hindu holiday observed each autumn in India, where it is known as the ‘celebration of lights’. Burning of fireworks on this day leads to air and noise pollution, causing adverse effects to human health. Objectives. To monitor and analyze air quality and noise levels in a residential college campus in northeast India over Diwali 2015. Methods. Components of PM10, including metals (cadmium (Cd), cobalt (Co), iron (Fe), zinc (Zn) and nickel (Ni)), ions (calcium (Ca2+), ammonium (NH4+), sodium (Na+), potassium (K+), chloride (Cl−), nitrate (NO3−) and sulfate (SO4 2−)) and bacterial counts were studied for a period of ten days in November 2015. In addition, a health-based survey of patients attending the institute's hospital during those days was conducted to evaluate the risk level due to fireworks burning. Results. The mean PM10 concentration during Diwali, 311 μg/m3, was 81% higher than other days and 3.1-times higher the Indian National Ambient Air Quality Standards. While noise levels were increased by 65%, the concentration of bacteria in PM10 was reduced by 39% during Diwali compared to other days. The concentrations of metals, cations and anions were increased by 51%, 72% and 77%, respectively. A health study conducted during the analysis period revealed an increase in hospital admissions in the campus due to respiratory symptoms. The higher concentrations of metals during the Diwali period resulted in a 0.5% increase in the hazard index. Conclusions. The present study suggests that reducing fireworks during Diwali could reduce pollutant concentrations and result in potential health benefits. Participant Consent. Obtained Ethics Approval. The study and survey design were approved by the Institutional Bioethics Committee of the Indian Institute of Technology, Guwahati. Competing Interests. The authors declare no competing financial interests


Introduction
Concentrations of regulated air pollutants exceed Indian National Ambient Air Quality Standards (NAAQS) in most cities in India. 1,2 Burning of fireworks leads to the release of large amounts of harmful gases and toxic substances into the atmosphere, thereby contaminating the air, causing adverse effects to human health and worsening air pollution. A rapid increase in pollutant concentrations due to fireworks has been previously reported. 3 Determination of PM 10 (particulate matter with aerodynamic diameter < 10 µm) in ambient air is essential, as pollution due to fireworks causes pulmonary effects, with greater impacts to the health of young children, elderly persons and pregnant women. 4,5 Fireworks are made up of various organic and inorganic chemicals which create smoke plumes that contain charcoal, sulfur, potassium, lead, aluminum, iron and barium nitrate. 6,7 Chemical reactions propel fireworks into the air and cause them to explode. While sodium (Na + ) and potassium (K + ) are used as metal oxidizers, zinc (Zn) is used to produce smoke effects and strontium (Sr) is used to stabilize fireworks. 7 Pollutants released from fireworks at a higher altitude are diluted before coming into contact with human populations, which can reduce health impacts. 8 However, ground level firework displays have an immediate impact on human health. Acute eosinophilic pneumonia has been reported in a patient due to inhalation of smoke continuously for three nights from burning of fireworks. 9 While fever, cough and dyspnoea are often reported initially as acute effects, pollution due to fireworks causes chronic respiratory and cardiovascular disease, pulmonary effects, premature death and cancer. 10-12 A 30% to 40% Background. Diwali is a Hindu holiday observed each autumn in India, where it is known as the 'celebration of lights' . Burning of fireworks on this day leads to air and noise pollution, causing adverse effects to human health. Objectives. To monitor and analyze air quality and noise levels in a residential college campus in northeast India over Diwali 2015. Methods. Components of PM 10 , including metals (cadmium (Cd), cobalt (Co), iron (Fe), zinc (Zn) and nickel (Ni)), ions (calcium (Ca 2+ ), ammonium (NH 4+ ), sodium (Na + ), potassium (K + ), chloride (Cl -), nitrate (NO 3-) and sulfate (SO 4 Research increase in the incidence of wheezing, respiratory disease, exacerbation of bronchial asthma and bronchitis has been reported during a Diwali celebration in India. 3 Several studies have been carried out in different parts of India to assess the impact of fireworks on PM 10 -related air quality. 11 A short-term study reported a 2-3 times elevation in PM 10 concentrations during a Diwali festival in Hisar city in northwest India. 13 PM 10 during Diwali in Lucknow in northern India was reported to be 7.53-times higher than on other days. 12 A five-fold increase in PM 10 concentration was observed in Kolkata in eastern India during Diwali. 14 A 35-fold increase in PM 10 concentration during Diwali was reported in Vadodara in western India due to burning of fireworks. 11 In Rajnandgaon in central India, PM 10 concentration during Diwali showed a nearly 3-fold increase compared to other days. 15 A 4-to 10-fold increase in PM 10 concentration was observed during Diwali in Nagpur in central India. 16 Owing to already high PM 10 concentrations, several studies were conducted in the national capital, New Delhi. High concentrations of PM 10 (767 μg/m 3 and 620 μg/m 3 ) were observed during the Diwali festivals of 2008 and 2009, respectively. 17 A community-based health survey conducted during Diwali in 2013 revealed an increase in the number of patients in Delhi with problems related to respiratory diseases, hearing issues, eye irritation and headache. 10 A previous study in northeast India observed an increase in the concentrations of metals, anions and cations during festival days compared to other days. 18 In Raipur, ion concentrations were 10 times greater than other days. 19 A similar increase in the metals associated with fireworks burning were reported in Delhi. 20 The prevalence of several respiratory symptoms such as asthma, inflammation, irritation, and fatigue due to bioaerosols have been previously reported. 21, 22 However, studies have observed a negative correlation between bacteria and heavy metals. 23 Therefore, along with chemical analysis, characterization of bioaerosols is helpful to determine the impact of heavy metals released due to burning of fireworks on bacterial activity.
In addition to air quality, noise levels are also affected by fireworks burning during Diwali, causing interference in communication and adverse health effects. 4 In Uttarakhand, 29.6% and 18.1% increases in average noise level on festival days compared to non-festival days were recorded in residential and commercial areas, respectively. 24 In Chennai, noise levels were considerably higher in residential areas during the Diwali festival. 25 Only two studies have been reported from northeast India. In Tezpur, a mean PM 10 concentration of 87.45 μg/ m 3 was observed during festival days, which was 2.13 times greater than normal day concentrations. 18 Another study from Dibrugarh reported PM 10 and PM 2.5 concentrations during Diwali of 168 and 160 μg/ m 3 , respectively, which was 5.33 and 5.74 times higher than normal day concentrations. 26 However, no examination of health correlations or risk levels was carried out in those studies.
Over the years, celebrations of Diwali have grown. However, there have been few studies examining the direct environmental impact of fireworks on the health of residents in a residential campus. Moreover, no investigations have been carried out in the biggest city of northeast India i.e. Guwahati, which has severe air quality issues. The main aim of the present study is to assess PM 10 and its associated health risk on residents during Diwali, along with noise levels and biological characteristics of PM 10 .

Methods
The present study was conducted at the Indian Institute of Technology (IIT) Guwahati. Guwahati is one of the fastest growing cities in India and the largest city in northeast India. The institute is located 20 km from the heart of the city. Guwahati is located in Assam, in northeast India, as shown in Figure 1. The city experiences a significant monsoon period, with cold, dry winters from late November to February. 27 The sampling location is close to an industrial region with an export promotion industrial park, Guwahati biotech park, skin and health care industry, pharmaceutical company, machine industry and a liquid petroleum gas bottling plant. Moreover, construction activities are prevalent throughout the year due to capacity expansion within the premises of the residential area. Vehicle traffic is mainly comprised of motorcycles and passenger cars, with

Sample collection
A fine particulate sampler, Envirotech APM 550, with an average flow rate of 16.7 L/min, was used to measure PM 10 concentrations. This instrument works on the principle of impaction to separate particles greater than 10 µm aerodynamic diameter. Filters with 47-mm diameter Whatman® quartz membrane filters and pore size of 0.2 μm were used to collect the samples. The same filters were analyzed for PM 10 and all other chemical and biological analyses by dividing the sample filter into aliquot parts. For the quality check, filters were sterilized by autoclaving at 121°C (250°F) and 100 kPa (15 psi) above atmospheric pressure for 15 minutes to ensure zero microorganisms before use in the sampler. Filters were carried to and from the site in sealed polyethylene bags for gravimetric analysis and utmost care was taken to avoid handling errors. For further quality assurance, blank samples were analyzed, and necessary corrections were included. Filter papers were desiccated for 24 hours before and after sampling to minimize errors due to moisture and achieve accurate measurements. Samples were weighed with an electronic balance (Model-Kerb & Sohn GmbH) with a sensitivity of 0.1 mg. To prevent manual contamination, a few drops of ethanol were applied to hands during filter placement and retrieval.
Noise level was measured using a sound level meter (Amprobe SM -20 A) at a height of 3-4 m from ground level, following Indian Central Pollution Control Board guidelines. 28 A four-day monitoring program was carried out at night during large fireworks displays, from November 11-14, 2015.
Weather data were collected using a weather station (Vantage Pro2) to collect parameters such as temperature, dew point, relative humidity, wind speed and wind direction. This weather station recorded data continuously and the data for each day were retrieved separately. Wind speed measurements from the ground and other obstructions were made at an approximate height of 5 m from ground level.
Sampling was conducted from November 8-17, 2015, to predict air quality variation. Samples collected on November 10, 11 and 12 were treated as Diwali samples. The samples before and after the Diwali period were treated as pre-and post-Diwali samples.
A health-based survey of patients attending the institute's hospital during those days was conducted (Supplemental Material). Information regarding age, body weight and various symptoms such as headache, fatigue, irritation, coughing, sneezing

Figure 1 -(a) Guwahati city in India; (b) Study area -Indian Institute of Technology Guwahati
Research and sinusitis experienced by patients were documented. A total of 350 patients were surveyed, out of which 322 participants with comprehensive information were considered. Patients who had already filled out the forms and returned the next day without any added symptoms were excluded. The chosen 322 participants reported their symptoms on the survey forms issued at the reception center. All the symptoms reported by patients were self-diagnosed. Most participants experienced fatigue or irritation and hence these symptoms were chosen as the limiting threshold for a positive response. However, the response rate was consistent throughout the study period, since this survey was first of its kind for all the residents in the campus.
This information was utilized for further study analyses such as risk level calculation. Participants were informed of the study parameters and assured of the confidentiality of disclosed personal information prior to their participation. The study and survey design were approved by the Institutional Bioethics Committee of the Indian Institute of Technology, Guwahati.
The sampling schedule and meteorological parameters recorded during the study period are shown in Table 1 where a mixture of 1.7 mmol/L nitric acid and 0.7 mmol/L dipicolinic acid was used as eluent with a flow rate of 0.9 mL/min. Twenty (20) μL of sample was measured using inbuilt loop and injected in to the ion chromatograph system. 14 Calibration and quantification of components were performed using Merck reference

Biological analysis
The aliquot (1/4th part) of PM 10 sample was dipped in the reagent bottle with sterilized distilled water of 10 mL. The serial dilution technique was followed, and the sample was poured on the petri plate with agar.

Research
Bacteria were allowed to grow on nutrient agar medium for 2-3 days incubated at 37ºC for all of the samples. 30 Finally, number of bacteria was quantified and reported as CFU/m 3 . Figure 2 shows the variation of PM 10 concentrations during pre-Diwali, Diwali and post-Diwali days. As seen in the figure, PM 10 concentrations always exceeded the 24-hour Indian NAAQS limit of 100 μg/m 3 . The mean concentration during non-Diwali days was 172 μg/m 3 , which were greater than the 24-hour Indian NAAQS limit set by Central Pollution Control Board (CPCB) indicating severe air pollution during fireworks burning. 48

Noise levels
The ambient noise level measured during Diwali was 101 dBA. The mean noise level of non-Diwali days was 61 dBA, which is 40 dBA less than during Diwali. In both the cases, the ambient noise level has crossed over the NAAQS limit of 55 dBA, in residential area during day time. 48

Biological analysis
Variation in bacteria concentrations are shown in Figure 3. Bacteria concentrations and ratios of bacteria and PM 10 concentrations were lower during Diwali compared to pre-and post-Diwali days. This could be microorganism inhibition due to heavy metals released in the atmosphere by burning of fireworks. Even though fireworks contain a high mass fraction of carbon content, the major ingredients are metal salts. The metal concentrations in the present study, except for Fe and Zn, were higher than in other studies.

Concentrations of six metals during
Diwali, and pre-and post-Diwali days are shown in Figure 4.

Figure 4 -Concentrations (µg/m 3 ) of metals (Zn, Fe, Cd, Co, Ni and Sr) during pre-Diwali, Diwali and post-Diwali days. Diwali/non-Diwali day ratios of those metals are indicated by a filled circle using the right y-axis.
Research compared to other studies around the world. In the present study, there was a decreasing order of anions and cations of Cl -> SO 4 2-> NO 3-and K + > NH 4+ > Na + > Ca 2+ , respectively, during the monitoring campaign. In comparison with other studies in this region, concentrations of studied ions were higher. The study location is a city with more pollution sources, leading to higher PM 10 concentrations than other studies. Figure 5 depicts the percentage variations of water-soluble ionic species collected during pre-Diwali, Diwali and post-Diwali periods.

Acidity/basicity of PM 10
The anion to cation (A/C) ratio gives an insight to the acidic or basic nature of aerosols. In order to calculate ion balance, mass concentrations of ions were converted to micro equivalents (µeq), as shown in Equation 1. 32 Figure 6 shows the graphical representation of A/C ratios for the Diwali period along with pre-and post-Diwali periods. An A/C ratio greater than 1 indicates the particle's acidic nature, whereas a ratio slightly less than 1 indicates the contribution of unmeasured carbonate ions. 32,33 A low A/C ratio indicates the basic nature of aerosols. 34

Health survey and assessment of health risk due to heavy metals
Panel (a) in Figure 7 indicates that the number of patients reporting headache, fatigue, irritation in eyes, coughing, sneezing and sinusitis increased by 3.8-, 3-, 3.3-, 3.3-, 3.5and 6.5-times, respectively, on Diwali

Figure 5 -Percentage variation of ions in PM 10 during pre-Diwali, Diwali and post-Diwali days
Research days compared to non-Diwali days. Similar findings have been found in a post-Diwali morbidity survey conducted in Delhi. 10 In the present study, five major metals (cadmium (Cd), cobalt (Co), iron (Fe), Zn and nickel (Ni)) were used to assess the possible noncarcinogenic human health risk due to this pollution episode. Two scenarios were taken into consideration. In scenario I, the cumulative hazard index (HI) due to metals (Cd, Co, Fe, Zn and Ni) for an annual period (350 days) was estimated using average concentrations during non-Diwali days. For this calculation, 350 days was based on United States Environmental Protection Agency (USEPA) guidelines which describes this value as the most frequent exposure that is reasonably expected at a site with two weeks of vacation. 35 The degree of over or under estimation was considered to be negligible.
Scenario II involved two parts (a) and (b). In II (a), the HI was calculated for all the metals for 347 days, excluding the Diwali period, by utilizing concentrations as in scenario I. In II (b), the HI for the Diwali period was calculated. Summation of II (a) and (b) was compared with the HI from Scenario I to determine the excess risk due to Diwali.
The non-carcinogenic health risk to an individual due to exposure to all metals 'j' is given by Equation 2: 20

Equation 2
where, RfC is the inhalation chronic reference concentration (mg/m 3 ) obtained from the Risk Assessment Information System (RAIS), M is

Garaga, Kota
Characterization of PM 10 and Impact on Human Health During the Annual Festival of Lights (Diwali)  4 2through gas phase oxidation by hydroxyl radicals or aqueous phase oxidation involving hydrogen peroxide, ozone, and other oxidants. 14, 40 In addition, sulphate salts are mainly used as coloring agents in fireworks, which may be the reason behind higher concentrations of sulphate in PM 10 .

Figure 7 -(a) Number of patients reporting headache, fatigue, irritation, coughing, sneezing and sinusitis during Diwali and Non-Diwali days, and (b) Incremental risk (%) due to Diwali from exposure to metals
In the present study, during non-Diwali days, the average A/C ratio was found to be 0.45, half the ration found on Diwali days (0.9). Similar observations were made by an air quality study conducted in residential sites in Bhilai. 19 This indicates that fireworks burning resulted in a more acidic particle composition.
The estimated incremental risk was highest for Zn (1.013), followed by Co (1.005), Sr (1.004) and the remaining elements Ni (1.0017), Fe (1.0016) and Cd (1.0011). The results indicated that the cumulative HI for Scenario II, which includes the Diwali period, showed a risk level increase of ~ 0.5% in health effects due to chronic lifetime exposure to metals alone. It should be noted that this risk level rise was only due to metals, and in ambient settings the risk level will be higher due to chronic exposure to many other pollutants such as SO 2 , mono-nitrogen oxide species (NO x ) and elemental carbon released by fireworks.

Conclusions
In the present study, concentrations of metals and ions in PM 10 were measured for a period of ten days in 2015, coinciding with the Diwali fireworks festival in northeast India. The results indicated that while the concentration of metals increased by 51%, ions increased by 74%. PM 10 showed a shift from anion to cation deficiency during fireworks burning. Additionally, ambient noise levels measured during Diwali were 101 Research dB, 40 dB higher than levels on non-Diwali days. Furthermore, the mean concentrations of bacteria during Diwali days showed a 61% reduction compared to non-Diwali days.
The increased level of particulate matter corresponded with a 67% increase in the number of patients attending the hospital during the Diwali period. The chronic lifetime exposure to metals alone during Diwali increased the health risk level by 0.5%. This study stresses the importance of regulated and monitored fireworks burning in regions with a high population density. The present study is limited in external validity due to its relatively small sample size and short duration. However, this study suggested the possible impact of fireworks on human health. Future time series analyses (for example, studies done by Atkinson 41 and Sahu and Kota, 42 ) are needed using data collected from additional fireworks burning events to quantify the relationship between PM 10 concentrations and health risks. 41,42