A STUDY TO EVALUATE EFFICACY OF 810nm DIODE LASER IN THE MAINTENANCE OF DENTAL IMPLANTS: A PERI-IMPLANT SULCULAR FLUID (PISF) ANALYSIS Introduction: Biological Implant failures are primarily related to biofilm which can lead to peri-mucositis and further on, peri implantitis. 810nm Diode laser has an affinity for pigmented chromophores, so its use in the peri-implant sulcus has a significant bactericidal effect on the black pigmented anaerobes such as P.gingivalis. Therefore, it can be used to eliminate or reduce the bacterial count in the Peri- Implant sulcular fluid (PISF), thus increasing the life of the implants and reducing the chances of failure. Aim: To evaluate the efficacy of 810nm Diode laser for the maintenance of Dental Implants. Objectives: To use the Diode laser as a regular in-office tool for limiting the microbiological count in the Peri-Implant Sulcular Fluid (PISF) and thus increasing the implant life. Methods: 20 patients visiting the Department of Periodontology and Oral implantology were randomly selected for the study who have been undergoing Implant treatment. Peri-Implant sulcular Fluid samples were collected before and after the sulcus was lased with 810nm Diode laser and sent for Quantitative Microbiological Analysis using Universal Bacterial Count and quantity of P.gingivalis using Real Time PCR. Results: The mean value for the Total Microbial count before and after 810nm Diode application was 282.5 (±24.46) and 74.5 (±47.403). The mean value for the total P.gingivalis count before and after 810nm Diode laser was 4.76 (±1.5) and 3.6 (±1.1). Conclusion: The two main outcomes of the study after 810nm Diode laser was used around the implant were as follows: 1) Drastic reduction in the Total bacterial count. 2) Significant reduction in the P. gingivalis count as evaluated by Real time PCR.

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