The objective of the study was to identify the seroprevalence of anti-Toxoplasma gondii antibodies in sheep herds from 3 municipalities from Jalisco, Mexico, as well as estimate the association between seroprevalence and certain factors presents in the farms. In total, 12 sheep farms that maintain only hair breeds were included in the work. From these farms, 336 blood samples were collected, corresponding 324 to ewes and 12 rams. Serum samples were subjected to ELISA test, and the association between the frequency of antibodies and some potential risk factors was estimated. The overall seroprevalence to anti-T. gondii antibodies in the population studied was 17.8% (60/336; 95% confidence interval [C.I.] 14–22), all farms had positive animals, and the seroprevalence of antibodies ranged between 7 to 32%. Seroprevalence in specific municipalities was 18.7% in Lagos de Moreno, 17.8% in Encarnación de Díaz, and 16.9% in San Juan de los Lagos. Seroprevalence in ewes was 17.5% (57/324; 95% C.I. 13–22), and seroprevalence in rams was 25% (3/12; 95% C.I. 6–57), while among breeds it was 17.8% in Pelibuey (20/112; 95% C.I. 11–26), 16.6% in Kathadin (14/84; 95% C.I. 9–26), 15.4% in Blackbelly (13/84; 95% C.I. 8–25), and 23.2% in Dorper (13/56; 95% C.I. 13–36); no differences were observed among breeds (p < 0.05). The presence of cats on the farms was associated with seroprevalence (odds ratio [OR] 2.8; 95% C.I. 1.8–7.3, p < 0.001), as was the absence of a rodent-control program (OR 1.5; 95% C.I. 0.8–3.2, p < 0.05). No other factors were identified as associated with seroprevalence.