Hookworm infection continues to be a serious problem in rural areas of China. Rapid reinfection and high cost limit the effectiveness of deworming programs. Vaccination offers an attractive alternative to mass chemotherapy. However, variation in vaccine antigens from field hookworm populations could conceivably limit efficacy of a vaccine developed from laboratory strains. Reported here are initial experiments to ascertain levels of molecular variation in a promising vaccine antigen, ASP-1, from the dog hookworm Ancylostoma caninum. ASP-1 from a Chinese strain of A. caninum was isolated from a third-stage larval cDNA library and compared to ASP-1 from a U.S. strain. There was 97% and 98% similarity in the DNA and amino acid sequences, respectively. There were 42 polymorphic sites between the sequences, 30 of which were synonymous. The 12 nonsynonymous substitutions resulted in 10 changes in the deduced amino acid sequence. Five of the amino acid changes were in the N-terminal domain, whereas the C-terminal domain was more highly conserved, containing only 2 amino acid changes. The results suggest that the effect of molecular variation in antigens from geographically separated parasite populations should be considered during vaccine development
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February 2000
Research Article|
February 01 2000
Variation between ASP-1 Molecules from Ancylostoma caninum in China and the United States
Shan Qiang;
Shan Qiang
aInstitute of Parasitic Diseases, Chinese Academy of Preventative Medicine, Shanghai 200025, China
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Zhan Bin;
Zhan Bin
aInstitute of Parasitic Diseases, Chinese Academy of Preventative Medicine, Shanghai 200025, China
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Xiao Shu-hua;
Xiao Shu-hua
aInstitute of Parasitic Diseases, Chinese Academy of Preventative Medicine, Shanghai 200025, China
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Feng Zheng;
Feng Zheng
aInstitute of Parasitic Diseases, Chinese Academy of Preventative Medicine, Shanghai 200025, China
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Peter Hotez;
Peter Hotez
bMedical Helminthology Laboratory, Department of Epidemiology and Public Health, Yale School of Medicine, New Haven, Connecticut 06520
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John M. Hawdon
John M. Hawdon
bMedical Helminthology Laboratory, Department of Epidemiology and Public Health, Yale School of Medicine, New Haven, Connecticut 06520
cMedical Helminthology Laboratory, Department of Epidemiology and Public Health, Yale School of Medicine, New Haven, Connecticut 06520;Corresponding author
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J Parasitol (2000) 86 (1): 181–185.
Citation
Shan Qiang, Zhan Bin, Xiao Shu-hua, Feng Zheng, Peter Hotez, John M. Hawdon; Variation between ASP-1 Molecules from Ancylostoma caninum in China and the United States. J Parasitol 1 February 2000; 86 (1): 181–185. doi: https://doi.org/10.1645/0022-3395(2000)086[0181:VBAMFA]2.0.CO;2
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