We report the detection and identification of Cryptosporidium and Giardia from 1 of 3 species of pinnipeds. Fecal samples were collected from Pacific harbor seal (Phoca vitulina richardsi), northern elephant seal (Mirounga angustirostris), and California sea lion (Zalophus californianus) in the northern California coastal area. By means of fluorescently labeled monoclonal antibodies, Cryptosporidium oocysts were detected in 3 samples from California sea lions, 1 of which also contained Giardia cysts. Oocysts of Cryptosporidium and cysts of Giardia were morphologically indistinguishable from oocysts of C. parvum and cysts of G. duodenalis from other animal origins. Oocysts and cysts were then purified using immunomagnetic separation techniques and identified by polymerase chain reaction (PCR), from which species-specific products were obtained. Sequence analysis revealed that the 452-bp and 358-bp PCR products of Cryptosporidium isolated from California sea lion had identities of 98% with sequences of their template fragments of C. parvum obtained from infected calves. Based on morphological, immunological, and genetic characterization, the isolates were identified as C. parvum and G. duodenalis, respectively. The findings suggested that California sea lions could serve as reservoirs in the environmental transmission of Cryptosporidium and Giardia.
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June 2000
LIFE CYCLES-SURVEY|
June 01 2000
FIRST FINDINGS OF CRYPTOSPORIDIUM AND GIARDIA IN CALIFORNIA SEA LIONS (ZALOPHUS CALIFORNIANUS)
Ming-Qi Deng;
Ming-Qi Deng
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California at Davis, One Shields Avenue, Davis, California 95616-8743
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Richard P. Peterson;
Richard P. Peterson
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California at Davis, One Shields Avenue, Davis, California 95616-8743
*California Department of Food and Agriculture, Animal Health Branch, Sacramento, California 95814.
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Dean O. Cliver
Dean O. Cliver
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California at Davis, One Shields Avenue, Davis, California 95616-8743
†To whom correspondence should be addressed.
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J Parasitol (2000) 86 (3): 490–494.
Citation
Ming-Qi Deng, Richard P. Peterson, Dean O. Cliver; FIRST FINDINGS OF CRYPTOSPORIDIUM AND GIARDIA IN CALIFORNIA SEA LIONS (ZALOPHUS CALIFORNIANUS). J Parasitol 1 June 2000; 86 (3): 490–494. doi: https://doi.org/10.1645/0022-3395(2000)086[0490:FFOCAG]2.0.CO;2
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