Reactivation of chronic toxoplasmosis resulting in Toxoplasma encephalitis (TE) is a common event in acquired immune deficiency syndrome (AIDS) patients. Conversion from Toxoplasma gondii bradyzoites to tachyzoites is a prerequisite for reactivation. Until recently, the study of stage conversion in human tissue was not possible due to the lack of antibodies that recognize stage-specific epitopes after long-term formaldehyde fixation. Using the combination of a polyclonal anti-T. gondii antibody, the cyst-stage-specific monoclonal antibody CC2, and a tachyzoite-specific polyclonal antibody (anti-SAG1, recombinant), we tried to demonstrate parasite differentiation in the brain tissue of 10 AIDS patients with clinically suspected TE. Double labeling of the stage-specific antibodies enabled us to demonstrate interconversion between tachyzoites and bradyzoites for the first time in human tissue. The study confirmed that the transformation process is nonsynchronous and that the manifestation of TE depends on the degree and site of tissue destruction caused by invading tachyzoites. The original source of tachyzoites could never be located, but a few samples suggested that tachyzoites may invade by dissemination across the blood–brain barrier. Cyst rupture as the first event in the process of reactivation was not seen. We conclude that the initial site(s) of reactivation will be destroyed by tissue-destructive tachyzoites long before clinical symptoms occur.
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June 2000
PATHOLOGY|
June 01 2000
IS STAGE CONVERSION THE INITIATING EVENT FOR REACTIVATION OF TOXOPLASMA GONDII IN BRAIN TISSUE OF AIDS PATIENTS?
Ingrid Reiter-Owona;
Ingrid Reiter-Owona
Institute of Medical Parasitology, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany
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Heike Seitz;
Heike Seitz
Institute of Medical Parasitology, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany
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Uwe Gross;
Uwe Gross
Institute of Medical Parasitology, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany
*Institute of Hygiene and Microbiology, University of Würzburg, Josef-Schneider Str. 2, 97080 Würzburg, Germany.
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Monika Sahm;
Monika Sahm
Institute of Medical Parasitology, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany
†Department of Ophthalmology, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.
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Jürgen K. Rockstroh;
Jürgen K. Rockstroh
Institute of Medical Parasitology, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany
‡Department of Medicine, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany.
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Hanns M. Seitz
Hanns M. Seitz
Institute of Medical Parasitology, University of Bonn, Sigmund-Freud-Str. 25, 53105 Bonn, Germany
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J Parasitol (2000) 86 (3): 531–536.
Citation
Ingrid Reiter-Owona, Heike Seitz, Uwe Gross, Monika Sahm, Jürgen K. Rockstroh, Hanns M. Seitz; IS STAGE CONVERSION THE INITIATING EVENT FOR REACTIVATION OF TOXOPLASMA GONDII IN BRAIN TISSUE OF AIDS PATIENTS?. J Parasitol 1 June 2000; 86 (3): 531–536. doi: https://doi.org/10.1645/0022-3395(2000)086[0531:ISCTIE]2.0.CO;2
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